Genome-wide CRISPR Screening to Identify Drivers of TGF-β-Induced Liver Fibrosis in Human Hepatic Stellate Cells

Autor:	Shan Yu, Matthew Ericson, Andrea Fanjul, Derek M. Erion, Maria Paraskevopoulou, Erin N. Smith, Banumathi Cole, Ryan Feaver, Corine Holub, Narender Gavva, Shane R. Horman, Jie Huang
Rok vydání:	2022
Předmět:	Liver Cirrhosis Liver Transforming Growth Factor beta Hepatic Stellate Cells Humans Molecular Medicine General Medicine Fibrosis Biochemistry Signal Transduction
Zdroj:	ACS Chemical Biology. 17:918-929
ISSN:	1554-8937 1554-8929
DOI:	10.1021/acschembio.2c00006
Popis:	Liver fibrosis progression in chronic liver disease leads to cirrhosis, liver failure, or hepatocellular carcinoma and often ends in liver transplantation. Even with an increased understanding of liver fibrogenesis and many attempts to generate therapeutics specifically targeting fibrosis, there is no approved treatment for liver fibrosis. To further understand and characterize the driving mechanisms of liver fibrosis, we developed a high-throughput genome-wide CRISPR/Cas9 screening platform to identify hepatic stellate cell (HSC)-derived mediators of transforming growth factor (TGF)-β-induced liver fibrosis. The functional genomics phenotypic screening platform described here revealed the novel biology of TGF-β-induced fibrogenesis and potential drug targets for liver fibrosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74b2f5e9de8fe7b0e417d602e2240b3a https://doi.org/10.1021/acschembio.2c00006 Zobrazit plný text záznamu