Neuropathic Pain Creates Systemic Ultrastructural Changes in the Nervous System Corrected by Electroacupuncture but Not by Pregabalin
| Autor: | Xi-Lai Xiao, Lei Gao, Jian-Xiong An, Jian-Feng Zhang, John P Williams, Xiao-Yan Qian, Wan-Rui Shi, Yi-Ning Yan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nervous system
neuropathic pain SNi structural changes Electroacupuncture business.industry medicine.medical_treatment Central nervous system Chronic pain Nerve injury medicine.disease Anesthesiology and Pain Medicine medicine.anatomical_structure Anesthesia Peripheral nervous system Neuropathic pain electroacupuncture medicine pregabalin medicine.symptom Journal of Pain Research business Original Research |
| Zdroj: | Journal of Pain Research |
| ISSN: | 1178-7090 |
| Popis: | Lei Gao,1,2,* Jian-Feng Zhang,3,* John P Williams,4 Yi-Ning Yan,1 Xi-Lai Xiao,1 Wan-Rui Shi,5 Xiao-Yan Qian,1 Jian-Xiong An1â 3,6 1Department of Anesthesiology, Pain and Sleep Medicine, Aviation General Hospital of China Medical University and Beijing Institute of Translational Medicine, Chinese Academy of Sciences, Beijing, Peopleâs Republic of China; 2School of Anesthesiology, Weifang Medical University, Weifang, Shangdong, Peopleâs Republic of China; 3Savaid Medical School, University of Chinese Academy of Sciences, Beijing, Peopleâs Republic of China; 4Department of Anesthesiology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; 5Department of Anesthesiology, Peking University Third Hospital, Beijing, Peopleâs Republic of China; 6School of Medical Science & Engineering, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, Peopleâs Republic of China*These authors contributed equally to this workCorrespondence: Jian-Xiong AnDepartment of Anesthesiology, Pain and Sleep Medicine, Aviation General Hospital of China Medical University, Beiyuan Road 3#, Beijing, 100012, Peopleâs Republic of ChinaTel +86 13801281750Fax +86 59520233Email anjianxiong@yeah.netPurpose: It is unclear whether neuropathological structural changes in the peripheral nervous system and central nervous system can occur in the spared nerve injury model. In this study, we investigated the pathological changes in the nervous system in a model of neuropathic pain as well as the effects of electroacupuncture (EA) and pregabalin (PGB) administration as regards pain relief and tissue repair.Patients and Methods: Forty adult male SD rats were equally and randomly divided into 4 groups: spared nerve injury group (SNI, n = 10), SNI with electroacupuncture group (EA, n = 10), SNI with pregabalin group (PGB, n =10) and sham-operated group (Sham, n=10). EA and PGB were given from postoperative day (POD) 14 to 36. EA (2 Hz and 100 Hz alternating frequencies, intensities ranging from 1â 1.5â 2 mA) was applied to the left âzusanliâ (ST36) and âYanglingquanâ (GB34) acupoints for 30 minutes. The mechanical withdrawal thresholds (MWTs) were tested with von Frey filaments. Moreover, the organizational and structural alterations of the bilateral prefrontal cortex, hippocampus, sciatic nerves and the thoracic, lumbar spinal cords and dorsal root ganglions (DRGs) were examined via light and electron microscopy.Results: MWTs of left hind paw demonstrated a remarkable decrease in the SNI model (P < 0.05). In the SNI model, ultrastructural changes including demyelination and damaged neurons were observed at all levels of the peripheral nervous system (PNS) and central nervous system (CNS). In addition, EA improved MWTs and restored the normal structure of neurons. However, the effect was not found in the PGB treatment group.Conclusion: Chronic pain can induce extensive damage to the central and peripheral nervous systems. Meanwhile, EA and PGB can both alleviate chronic pain syndromes in rats, but EA also restores the normal cellular structures, while PGB is associated with no improvement.Keywords: neuropathic pain, structural changes, electroacupuncture, pregabalin |
| Databáze: | OpenAIRE |
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