In vitro antifungal activity of Myracrodruon urundeuva Allemão against human vaginal Candida species
| Autor: | Vanessa C Rorato, Adriana Araújo de Almeida-Apolonio, Alexeia Barufatti Grisolia, Aline Lima de Barros, Allan Belarmino Rodrigues, Andréia Sangalli, Jonas da Silva Mota, Fernando A De Oliveira, Arielle Cristina Arena, Kelly Mari Pires de Oliveira |
|---|---|
| Přispěvatelé: | Fundacao Univ Fed Grande Dourados, Universidade Federal de Mato Grosso do Sul (UFMS), Universidade Estadual Paulista (Unesp), Universidade Estadual de Mato Grosso do Sul (UEMS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antifungal Agents Anacardiaceae Population Ethyl acetate Microbial Sensitivity Tests medicine.disease_cause traditional medicine 01 natural sciences 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound Candida albicans medicine Animals Humans Myracrodruon urundeuva education lcsh:Science Flavonoids education.field_of_study Multidisciplinary biology Traditional medicine Plant Extracts food and beverages biology.organism_classification candidiasis Acute toxicity 0104 chemical sciences Rats Plant Leaves 010404 medicinal & biomolecular chemistry 030104 developmental biology chemistry visual_art visual_art.visual_art_medium Plant Bark Bark Female lcsh:Q Tannins Genotoxicity Brazil |
| Zdroj: | Anais da Academia Brasileira de Ciências v.89 n.3 suppl.0 2017 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC Anais da Academia Brasileira de Ciências, Iss 0 Anais da Academia Brasileira de Ciências, Volume: 89, Issue: 3 Supplement, Pages: 2423-2432, Published: 24 JUL 2017 Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0001-3765 |
| Popis: | Made available in DSpace on 2018-11-26T17:42:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01. Added 1 bitstream(s) on 2021-07-15T14:32:07Z : No. of bitstreams: 1 S0001-37652017000502423.pdf: 590183 bytes, checksum: b16032f5533e8c13486b0d8851b77988 (MD5) Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Myracrodruon urundeuva is a plant native to Brazil, which is used by the indigenous population for the treatment of candidiasis. The aims of this study were to evaluate the antifungal activity of extract against human vaginal Candida species and evaluate the possible toxicological activities of M. urundeuva. Initially, ethanol extracts, ethyl acetate fractions, and hydroalcoholic fractions of the bark and leaf of M. urundeuva were used to determine the minimum inhibitory concentration. The extracts that showed antifungal activity were characterized by liquid chromatography and subjected to toxicity assessment. Toxic, cytotoxic, genotoxic, and mutagenic testing were performed using Allium cepa and Ames assays with the ethanol extracts of the bark and leaves. Hemolytic activity was evaluated in erythrocytes and acute toxicity in rats. The ethanol bark extracts showed best activity against Candida albicans, C. krusei, and C. tropicalis ATCC (4-512 mu g/mL). Chemical characterization indicated the presence of flavonoids and tannins in the extracts. Hemolytic activity, genotoxicity, and mutagenicity were not observed. The results of the Ames and A. cepa tests were also in agreement, ethanol bark extracts and ethanol leaf extracts of M. urundeuva showed absence of mutagenic activity. Similar results were observed in the A. cepa assay and acute toxicity test in rats. M. urundeuva bark extracts showed potential for the treatment of vaginal infections caused Candida species, as a topical. Fundacao Univ Fed Grande Dourados, Fac Ciencias Exatas & Tecnol, Rodovia Dourados Itahum,Km 12,Cidade Univ, BR-79804970 Dourados, MS, Brazil Fundacao Univ Fed Grande Dourados, Fac Ciencias Saude, Rodovia Dourados Itahum,Km 12,Cidade Univ, BR-79804970 Dourados, MS, Brazil Univ Fed Mato Grosso do Sul, Fac Med, Av Costa & Silva S-N,Cidade Univ, Campo Grande, MS 79070 USA Univ Estadual Paulista, Dept Morfol, R Luis Cassineli, BR-18618024 Botucatu, SP, Brazil Univ Estadual Mato Grosso do Sul, Dept Quim, Rodovia Dourados Itahum,Km 12,Cidade Univ, BR-79804970 Dourados, MS, Brazil Fundacao Univ Fed Grande Dourados, Fac Ciencias Biol & Ambientais, Rodovia Dourados Itahum,Km 12,Cidade Univ, BR-79804970 Dourados, MS, Brazil Univ Estadual Paulista, Dept Morfol, R Luis Cassineli, BR-18618024 Botucatu, SP, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|