Extracellular vesicles from women with breast cancer promote an epithelial-mesenchymal transition-like process in mammary epithelial cells MCF10A
| Autor: | Cristina Gonzales-Vazquez, Sonia Chávez-Ocaña, Eduardo Perez Salazar, Octavio Galindo-Hernandez, Emmanuel Reyes-Uribe, Monica Sierra-Martinez, Octavio D Reyes-Hernández, Pedro Cortes-Reynosa |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Epithelial-Mesenchymal Transition Angiogenesis Vimentin Breast Neoplasms Cell Line Extracellular Vesicles Plasma Breast cancer Downregulation and upregulation medicine Humans Epithelial–mesenchymal transition Mammary Glands Human biology Transition (genetics) General Medicine medicine.disease Neoplasm Proteins Gene Expression Regulation Neoplastic Tumor progression biology.protein Cancer research Female Extracellular Matrix Degradation |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(12) |
| ISSN: | 1423-0380 |
| Popis: | Extracellular vesicles (EVs) mediate many stages of tumor progression including angiogenesis, escape from immune surveillance, and extracellular matrix degradation. We studied whether EVs from plasma of women with breast cancer are able to induce an epithelial-mesenchymal transition (EMT) process in mammary epithelial cells MCF10A. Our findings demonstrate that EVs from plasma of breast cancer patients induce a downregulation of E-cadherin expression and an increase of vimentin and N-cadherin expression. Moreover, EVs induce migration and invasion, as well as an increase of NFκB-DNA binding activity and MMP-2 and MMP-9 secretions. In summary, our findings demonstrate, for the first time, that EVs from breast cancer patients induce an EMT-like process in human mammary non-tumorigenic epithelial cells MCF10A. |
| Databáze: | OpenAIRE |
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