Stability and validity of intact parathyroid hormone levels in different sample types and storage conditions

Autor: Ahmed A. Mirza, Fawzi F Bokhari, Anwar Borai, Mohammed Dakhakhni, Haitham Khalil, Hala Faizo, Suhad Bahijri, Gordon A. Ferns
Rok vydání: 2021
Předmět:
Zdroj: Journal of Clinical Laboratory Analysis
ISSN: 1098-2825
0887-8013
Popis: Background Several pre‐analytical factors can affect the measurement of intact Parathyroid Hormone (IPTH). In this study, we have investigated the effects of using different types of tubes, time elapsed before separation, and storage conditions over time on the measured values of IPTH. Method Blood samples from 30 subjects were collected into plain, SST, and EDTA tubes. All serum and plasma were separated immediately (first set) and after 2 hrs delay (second set). The first set of samples were aliquoted and stored at RT (25°C), at fridge (4°C), and freezer (−20°C). IPTH was measured in all the stored aliquots at 2,4, and 8 days after collection using Architect analyzer. Results Paired T test and ANOVA repeated measures showed no significant difference between IPTH levels in all tubes. The second set of serum and plasma were significantly lower (3.8% and 7.4%, p
Intact Parathyroid Hormone (IPTH) can be affected by different pre‐analytical factors. In this study, we investigated the effect of different types of tubes, delay in sample separation, and different storage temperature on the measured IPTH values. We found that plasma IPTH can be stored at RT for up to four days. Both plasma and serum IPTH are not affected by a delay in separation of up to two h and they can be stored for up to 8 days in a fridge or freezer without any clinically significant changes in their values.
Databáze: OpenAIRE
Externí odkaz: