Anti-microbial and Anti-oxidant Activity of Watermelon (Citrullus lanatus) Fruit and Watermelon Seed
| Autor: | Sevda Manap, Perihan Akbaş, Abdurrahman Gürbüz, Özlem Gürsoy Kol |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Citrullus lanatus
biology DPPH Mühendislik Rhodotorula biology.organism_classification Antimicrobial Xanthomonas campestris chemistry.chemical_compound anti-microbial anti-oxidant Citrullus lanatus watermelon watermelon seed Engineering chemistry Serratia marcescens Botany Materials Chemistry Butylated hydroxytoluene Food science Butylated hydroxyanisole |
| Zdroj: | Volume: 13, Issue: 1 139-147 Celal Bayar University Journal of Science |
| ISSN: | 1305-130X 1305-1385 |
| Popis: | Watermelon (Citrullus lanatus) flesh andseeds were dried and pulverized, and their extracts were diffused to sterilediscs for the evaluation of anti-microbial activity. The disc-diffusiontechnique was used to assess anti-bacterial activity against Bacillus subtilis, Escherichia coli,Pseudomonas aeruginosa, Pasteurella multocida, Yersinia enterocolitica, Serratia marcescens, Klebsiella pneumoniae, Xanthomonas campestris, Staphylococcus aureus. Anti-fungal activity against the yeasts Candida albicans and Rhodotorula glutinis was also examined.Standard anti-biotics were also tested as controls.Watermelon flesh andseedextractswere foundto be effective against gram-positive andgram-negativebacteria and yeasts. The extracts were also screened foranti-oxidant activity using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH)free-radical-scavenging assay, total reducing ability using the Fe3+–Fe2+transformation method and ferrous ion (Fe2+)-chelating activity.Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT),ethylenediaminetetraacetic acid and α-tocopherol were used as referenceanti-oxidant radical scavenger compounds. The most potent anti-bacterial activity wasdemonstrated by watermelon–ethanol extract (inhibition zone 30 mm) against K. pneumoniae, and the most potent anti-fungalactivity was demonstrated by watermelon–acetone extract (inhibition zone 26 mm)against R. glutinis. Watermelon–ethanoland watermelon seed–ethanol extracts both demonstrated marked anti-oxidantactivity.These results highlight that watermelon fruitand seed extracts have potential for the development of efficient, safe andcost-effective natural anti-oxidant compounds for application in the functionalfood industries. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|