Impact of Sampling and Cellular Separation on Amino Acid Determinations in Drosophila Hemolymph
| Autor: | Marissa R. Cabay, Scott A. Shippy, Jasmine C. Harris |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Model system Cell Separation 01 natural sciences Analytical Chemistry 03 medical and health sciences Hemolymph Animals Amino Acids Amino acid content Drosophila chemistry.chemical_classification Chemical content biology fungi 010401 analytical chemistry biology.organism_classification 0104 chemical sciences Amino acid Drosophila melanogaster 030104 developmental biology Biochemistry chemistry Composition (visual arts) Sample collection |
| Zdroj: | Analytical Chemistry. 90:4495-4500 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | The fruit fly is a frequently used model system with a high degree of human disease-related genetic homology. The quantitative chemical analysis of fruit fly tissues and hemolymph uniquely brings chemical signaling and compositional information to fly experimentation. The work here explores the impact of measured chemical content of hemolymph with three aspects of sample collection and preparation. Cellular content of hemolymph was quantitated and removed to determine hemolymph composition changes for seven primary amine analytes. Hemolymph sampling methods were adapted to determine differences in primary amine composition of hemolymph collected from the head, antenna, and abdomen. Also, three types of anesthesia were employed with hemolymph collection to quantitate effects on measured amino acid content. Cell content was found to be 45.4 ± 22.1 cells/nL of hemolymph collected from both adult and larvae flies. Cell-concentrated fractions of adult, but not larvae, hemolymph were found to have higher and more variable amine content. There were amino acid content differences found between all three areas indicating a robust method to characterize chemical markers from specific regions of a fly, and these appear related to physiological activity. Methods of anesthesia have an impact on hemolymph amino acid composition related to overall physiological impact to fly including higher amino acid content variability and oxygen deprivation effects. Together, these analyses identify potential complications with Drosophila hemolymph analysis and opportunities for future studies to relate hemolymph content with model physiological activity. |
| Databáze: | OpenAIRE |
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