Determination of acid alpha-naphthyl acetate esterase enzyme activity in peripheral blood leukocytes of gazelles (Gazella subgutturosa)
| Autor: | Hikmet Altunay, Nevin Kurtdede, R. N. Aştı, İsmail Şah Harem, Melek Koçak Harem |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
musculoskeletal diseases
Blood Platelets Histology Naphthol AS D Esterase Biology Gazella subgutturosa Eosinophilic Leukocytes Animals Platelet skin and connective tissue diseases chemistry.chemical_classification General Medicine Ruminants biology.organism_classification Molecular biology Enzyme assay Staining stomatognathic diseases Medical Laboratory Technology Enzyme chemistry Biochemistry Cytoplasm Alpha-naphthyl Acetate Esterase biology.protein |
| Zdroj: | Biotechnichistochemistry : official publication of the Biological Stain Commission. 83(6) |
| ISSN: | 1473-7760 |
| Popis: | We examined gazelle peripheral blood leucocytes using the alpha-Naphthyl acetate esterase (ANAE) staining technique (pH 5.8). Our purpose was to determine the percentage of ANAE positive lymphocytes. The proportion of ANAE positive T-lymphocytes was 72%. T-lymphocytes showed an ANAE positive reaction, but eosinophilic granulocytes and monocytes also showed a positive reaction. By contrast, no reaction was detected in B-lymphocytes, neutrophil granulocytes or platelets. The reaction observed in T-lymphocytes was a red-brown coloration, usually 1-2 granules, but enough granules to fill the cytoplasm were detected rarely. As a result of ANAE enzyme staining, we concluded that the staining technique can be used as a cytochemical marker for gazelle T-lymphocytes. |
| Databáze: | OpenAIRE |
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