Structure of the Oligosaccharide Chain of the SR-Type Lipopolysaccharide of Ralstonia solanacearum Toudk-2
| Autor: | Yuriy A. Knirel, Kerstin Wydra, Evelina L. Zdorovenko, B. Lindner, Evgeny V. Vinogradov |
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| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Spectrometry Mass Electrospray Ionization Magnetic Resonance Spectroscopy Polymers and Plastics Lipopolysaccharide Oligosaccharides Bioengineering Microbiology Biomaterials Hydrolysis chemistry.chemical_compound Materials Chemistry Ions chemistry.chemical_classification Ralstonia solanacearum Fourier Analysis biology Polysaccharides Bacterial O Antigens food and beverages biochemical phenomena metabolism and nutrition Oligosaccharide Chromatography Ion Exchange biology.organism_classification Models Chemical Biochemistry chemistry Chromatography Gel bacteria Acid hydrolysis Bacteria |
| Zdroj: | Biomacromolecules. 9:2215-2220 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/bm800326u |
| Popis: | Aiming at improving classification and taxonomy of Gram-negative phytopathogenic bacteria, we studied the structure of the lipopolysaccharide of Ralstonia solanacearum. Mild acid hydrolysis of the lipopolysaccharide of strain Toudk-2 followed by gel chromatography resulted in an O-polysaccharide and two oligosaccharide fractions. The smallest-size oligosaccharide fraction was studied by sugar analysis, high-resolution electrospray ionization mass spectrometry, and, after fractionation by anion-exchange chromatography on HiTrap Q, by one- and two-dimensional (1)H and (13)C NMR spectroscopy. It was found that the isolated oligosaccharides consist of the lipopolysaccharide core with one O-polysaccharide repeat (O-unit) attached. The core exists in two major glycoforms differing from each other in a lateral octulosonic acid residue, which is either D-glycero-D-talo-oct-2-ulosonic acid or 3-deoxy-D-manno-oct-2-ulosonic acid. A peculiar feature of the core is the occurrence of 4-amino-4-deoxy-L-arabinose nonstoichiometrically linked to a heptose residue. The full structures of the core and the biological O-unit as well as the site of the attachment of the O-unit to the core were established. |
| Databáze: | OpenAIRE |
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