Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis
| Autor: | Rubens Monti, Anna Paula Godoy, Fernando Masarin, Avelino Corma, Fernando Roberto Paz-Cedeno, Ricardo K. Donato, Jose Miguel Carceller, Sara Iborra, Ariela Veloso de Paula |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Institute of Chemical Technology (ITQ), Czech Academy of Sciences, Mackenzie Presbyterian University, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España) |
| Rok vydání: | 2021 |
| Předmět: |
Immobilized enzyme
020209 energy Lignocellulosic biomass Monomeric fermentable sugars 02 engineering and technology Cellulase chemistry.chemical_compound Hydrolysis QUIMICA ORGANICA 0202 electrical engineering electronic engineering information engineering Enzyme immobilization 0601 history and archaeology Ethanol fuel Cellulose Graphene oxide 060102 archaeology biology Renewable Energy Sustainability and the Environment Chemistry Sugarcane bagasse hydrolysis 06 humanities and the arts Cellulosic ethanol Magnetic nanoparticles biology.protein Biocatalyst Bagasse Nuclear chemistry |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 0960-1481 |
| DOI: | 10.1016/j.renene.2020.09.059 |
| Popis: | [EN] For producing second-generation ethanol (cellulosic ethanol) and other value-added bioproducts, magnetic graphene oxide (GO-MNP) was synthesized in this work and used as the immobilization support for an industrial cellulase-and xylanase-containing preparation. GO-MNP characterization by TEM, SEM and ATR-FTIR spectroscopy showed that the magnetic nanoparticles are homogeneously distributed onto the GO sheets surface. The enzymatic preparation was immobilized by means of carbodiimide cross-linking chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (NHS). The supported final biocatalyst (GO-MNP-Enz) showed high activity for the hydrolysis of pretreated sugarcane bagasse (PSB) and presented relative endoglucanase, xylanase, beta-glucosidase, and beta-xylosidase activities of 70%, 66%, 88%, and 70%, respectively, after 10 cycles of hydrolysis of their respective substrates. The biocatalyst also maintained approximately 50% and 80% of its efficiency for cellulose and xylan hydrolysis, respectively, being the TOF (g.g(-1).h(-1)) the highest observed when compared with previous results reported in literature. These findings suggest that GO-MNP-Enz may be a prospective candidate for industrial applications such as second-generation ethanol production. (C) 2020 Elsevier Ltd. All rights reserved. Sao Paulo State Research Support Foundation (FAPESP) contract number 2018/06241e3 funding this work. Coordination of Improvement of Higher Education Personnel (CAPES) funding the doctoral scholarship of Fernando Roberto Paz-Cedeno in Brazil and in the Universitat Politecnica de Valencia (UPV), Institute of Chemical Technology (ITQ), Valencia, Spain. Authors acknowledge financial support from PGC2018-097277-B-100 (MCIU/AEI/FEDER,UE) project and Severo Ochoa Program (SEV-2016-0683). |
| Databáze: | OpenAIRE |
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