Desipramine and citalopram attenuate pretest swim-induced increases in prodynorphin immunoreactivity in the dorsal bed nucleus of the stria terminalis and the lateral division of the central nucleus of the amygdala in the forced swimming test
Autor: | Hyun Ju Kim, Jin Hee Cho, Yun Ha Cho, Sun Hye Choi, Hee Jeong Kim, Kyung Ho Shin, Dong Hoon Kim, Sung Chung |
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Rok vydání: | 2014 |
Předmět: |
Male
medicine.medical_specialty Dynorphin Citalopram Nucleus accumbens behavioral disciplines and activities Amygdala Mice Cellular and Molecular Neuroscience Endocrinology Desipramine Internal medicine medicine Animals RNA Messenger Phosphorylation Protein Precursors Cyclic AMP Response Element-Binding Protein Dorsomedial hypothalamic nucleus Swimming Mice Inbred ICR Endocrine and Autonomic Systems Chemistry Central nucleus of the amygdala Central Amygdaloid Nucleus Enkephalins General Medicine Immunohistochemistry Antidepressive Agents Stria terminalis medicine.anatomical_structure Neurology Septal Nuclei human activities Stress Psychological Behavioural despair test medicine.drug |
Zdroj: | Neuropeptides. 48:273-280 |
ISSN: | 0143-4179 |
Popis: | Dynorphin in the nucleus accumbens shell plays an important role in antidepressant-like effect in the forced swimming test (FST), but it is unclear whether desipramine and citalopram treatments alter prodynorphin levels in other brain areas. To explore this possibility, we injected mice with desipramine and citalopram 0.5, 19, and 23 h after a 15-min pretest swim and observed changes in prodynorphin expression before the test swim, which was conducted 24 h after the pretest swim. The pretest swim increased prodynorphin immunoreactivity in the dorsal bed nucleus of the stria terminalis (dBNST) and lateral division of the central nucleus of the amygdala (CeL). This increase in prodynorphin immunoreactivity in the dBNST and CeL was blocked by desipramine and citalopram treatments. Similar changes in prodynorphin mRNA levels were observed in the dBNST and CeL, but these changes did not reach significance. To understand the underlying mechanism, we assessed changes in phosphorylated CREB at Ser(133) (pCREB) immunoreactivity in the dBNST and central nucleus of the amygdala (CeA). Treatment with citalopram but not desipramine after the pretest swim significantly increased pCREB immunoreactivity only in the dBNST. These results suggest that regulation of prodynorphin in the dBNST and CeL before the test swim may be involved in the antidepressant-like effect of desipramine and citalopram in the FST and suggest that changes in pCREB immunoreactivity in these areas may not play an important role in the regulation of prodynorphin in the dBNST and CeA. |
Databáze: | OpenAIRE |
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