An in vitro long-term study of cryopreserved umbilical cord blood-derived platelet-rich plasma containing growth factors-PDGF-BB, TGF-β, and VEGF
| Autor: | Yasuhito Shimakura, Yasuharu Yamazaki, Akira Takeda, Kazuno Moriyama, Yoshika Sugimoto, Yumiko Sone, Kennichi Kumazawa, Takayuki Sugimoto, Kyoko Baba |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_treatment Becaplermin ALIZARIN RED Umbilical cord Cryopreservation Umbilical Cord Andrology 03 medical and health sciences chemistry.chemical_compound fluids and secretions 0302 clinical medicine Transforming Growth Factor beta medicine biology business.industry Platelet-Rich Plasma Growth factor 030206 dentistry Fetal Blood Vascular endothelial growth factor medicine.anatomical_structure Otorhinolaryngology chemistry 030220 oncology & carcinogenesis Platelet-rich plasma embryonic structures biology.protein Surgery Oral Surgery business Platelet-derived growth factor receptor Transforming growth factor |
| Zdroj: | Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery. 47(4) |
| ISSN: | 1878-4119 |
| Popis: | Purpose Umbilical cord blood-derived platelet-rich plasma (UCB-PRP) containing growth factors has attracted attention as a biomaterial useful for regenerative medicine. The osteoblastic differentiation of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) can be induced by UCB-PRP. Materials and methods Nine samples of UC and UCB were used to conduct an in vitro study that determined the contents of three growth factors (i.e., platelet-derived growth factor, transforming growth factor β-1, and vascular endothelial growth factor) and that examined, by staining with Alizarin red, their ability to induce the osteoblastic differentiation of UC-MSCs at the baseline, 3 months, and 3 years of cryopreservation. Results The contents of growth factors in cryopreserved UCB-PRP were markedly elevated compared to those found in UCB at baseline. The samples of UCB that were added with cryopreserved UCB-PRP and those with bone morphogenetic protein-2 were stained granularly with Alizarin red, thus indicating the presence of calcium. The samples of UCB that were not added with UCB-PRP were not stained with Alizarin red. The above-mentioned contents and ability were maintained at 3 years of cryopreservation. Cryopreserved UCB-PRP possibly and advantageously induced the osteoblastic differentiation of UC-MSCs. Conclusion The potential clinical application of cryopreserved UCB-PRP to regenerative medicine was suggested. |
| Databáze: | OpenAIRE |
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