Fluorescent angioscopic imaging of calcium phosphate tribasic: precursor of hydroxyapatite, the major calcium deposit in human coronary plaques

Autor: Seiichiro Shirai, Osamu Nakagawa, Ei Shimoyama, Takanobu Kobayashi, Yasumi Uchida, Nobuyuki Hiruta
Rok vydání: 2017
Předmět:
Calcium Phosphates
Male
0301 basic medicine
Pathology
Color fluorescent angioscopy
Angioscopy
Coronary Artery Disease
030204 cardiovascular system & hematology
chemistry.chemical_compound
0302 clinical medicine
Von Kossa stain
medicine.diagnostic_test
Optical Imaging
Human coronary plaques
Middle Aged
Coronary Vessels
Fluorescence
Plaque
Atherosclerotic
Calcium phosphate tribasic
medicine.anatomical_structure
Female
Autopsy
Cardiology and Cardiovascular Medicine
Methylene blue
medicine.medical_specialty
chemistry.chemical_element
Calcium
Stain
Collagen Type I
Necrosis
03 medical and health sciences
Predictive Value of Tests
medicine
Humans
Oil Red O
Radiology
Nuclear Medicine and imaging
Vascular Calcification
Aged
Fluorescent Dyes
Original Paper
Lac dye
business.industry
Surgery
Coronary arteries
Durapatite
030104 developmental biology
chemistry
Luminescent Measurements
business
Azo Compounds
Biomarkers
Zdroj: The International Journal of Cardiovascular Imaging
ISSN: 1573-0743
1569-5794
DOI: 10.1007/s10554-017-1142-y
Popis: Coronary calcification is a risk factor for ischemic heart disease. Hydroxyapatite that is formed by polymerization from calcium phosphate tribasic (CPT) is the major constituent of coronary calcium deposits. If CPT could be visualized, coronary calcification could be predicted and prevented. We discovered that when CPT and collagen I, the main constituent of collagen fibers, are mixed with lac dye (LD) and then exposed to fluorescent light excited at 345 ± 15 nm and emitted at 420 nm, a purple fluorescence that is characteristic of CPT only is elicited. So, we examined localization of CPT and its relation to plaque morphology by color fluorescent angioscopy (CFA) or microscopy (CFM) in 24 coronary arteries obtained from 12 autopsy subjects. By CFA, the incidence (%) of CPT as confirmed by purple fluorescence in 15 normal segments, 25 white plaques, 14 yellow plaques without necrotic core (NC) and 8 yellow plaques with NC was 20, 36, 64 and 100 (p
Databáze: OpenAIRE
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