Evaluation of the articular cartilage in the knees of rats with induced arthritis treated with curcumin

Autor: Jean Faber, Diogo Correa Maldonado, Marcelo Cavenaghi Pereira da Silva, Tiago Nicoliche
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Cartilage
Articular
Male
Knee Joint
Physiology
Arthritis
Articular cartilage
Osteoarthritis
Injections
Intra-Articular
chemistry.chemical_compound
0302 clinical medicine
Mathematical and Statistical Techniques
Animal Cells
Immune Physiology
Medicine and Health Sciences
Connective Tissue Cells
Statistical Data
Principal Component Analysis
Innate Immune System
Multidisciplinary
Statistics
Treatment period
medicine.anatomical_structure
Connective Tissue
030220 oncology & carcinogenesis
Physical Sciences
Immunohistochemistry
Cytokines
Medicine
Anatomy
Cellular Types
Research Article
Statistical Distributions
medicine.medical_specialty
Curcumin
Science
Immunology
Research and Analysis Methods
03 medical and health sciences
Chondrocytes
Rheumatology
Internal medicine
medicine
Animals
Statistical Methods
Rats
Wistar
Collagen Type II
business.industry
Cartilage
Zymosan
Biology and Life Sciences
Cell Biology
Molecular Development
medicine.disease
Probability Theory
Rats
030104 developmental biology
Endocrinology
Biological Tissue
chemistry
Immune System
Multivariate Analysis
business
Mathematics
Articular Cartilage
Developmental Biology
Zdroj: PLoS ONE, Vol 15, Iss 3, p e0230228 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: This study was designed to evaluate the anti-inflammatory effects of a curcumin treatment on the knee of rats with induced osteoarthritis. Fifteen adult rats were used and divided in three groups: the osteoarthritis group (OAG), control group (CG–without induction of osteoarthritis), and curcumin-treated osteoarthritis group (COAG). Osteoarthritis was induced in the right knee of rats in the OAG and COAG by administering an intra-articular injection of 1 mg of zymosan. Fourteen days after induction, 50 mg/kg curcumin was administered by gavage daily for 60 days to the COAG. After the treatment period, rats from all groups were euthanized. Medial femoral condyles were collected for light microscopy and immunohistochemical staining. The expression of SOX-5, IHH, MMP-8, MMP-13, and collagen 2 (Col2) was analyzed. The COAG exhibited an increase in the number of chondrocytes in the surface and middle layers compared with that of the OAG and CG, respectively. The COAG also showed a decrease in the thicknesses of the middle and deep layers compared with those of the OAG, and an increase in Col2 expression was observed in all articular layers (surface, middle, and deep) in the COAG compared with that in the OAG. SOX-5 expression was increased in the surface and deep layers of the COAG compared with those in the OAG and CG. Based on the results of this study, the curcumin treatment appeared to exert a protective effect on cartilage, as it did not result in an increase in cartilage thickness or in MMP-8 and MMP-13 expression but led to increased IHH, Col2, and SOX-5 expression and the number of chondrocytes.
Databáze: OpenAIRE
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