Tissue-Specific Knockdown of Genes of the Argonaute Family Modulates Lifespan and Radioresistance in Drosophila Melanogaster
| Autor: | Ilya Solovev, Elena Yushkova, Daria Yakovleva, Alexey Moskalev, Evgeniya Shchegoleva, Nadezhda Zemskaya, Ekaterina Proshkina, Natalya R. Pakshina, Natalia S. Ulyasheva, Liubov Koval, Mikhail Shaposhnikov |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Small RNA Longevity Piwi-interacting RNA Biology Radiation Tolerance Catalysis Article Inorganic Chemistry lcsh:Chemistry piwi RNA interference Radioresistance Gene silencing biochemistry Tissue specific Animals Drosophila Proteins Physical and Theoretical Chemistry RNA Small Interfering Molecular Biology Gene lcsh:QH301-705.5 Spectroscopy Agronaute Regulation of gene expression Gene knockdown Organic Chemistry aging General Medicine Argonaute biology.organism_classification Computer Science Applications Cell biology radioresistance Drosophila melanogaster lcsh:Biology (General) lcsh:QD1-999 Gamma Rays Organ Specificity Argonaute Proteins Female RNA Interference ionizing radiation lifespan |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 2396, p 2396 (2021) International Journal of Molecular Sciences Volume 22 Issue 5 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Small RNAs are essential for the coordination of many cellular processes, including the regulation of gene expression patterns, the prevention of genomic instability, and the suppression of mutagenic transposon activity. These processes determine aging, longevity, and sensitivity of cells and an organism to stress factors (particularly, ionizing radiation). The biogenesis and activity of small RNAs are provided by proteins of the Argonaute family. These proteins participate in the processing of small RNA precursors and the formation of an RNA-induced silencing complex. However, the role of Argonaute proteins in the regulation of lifespan and radioresistance remains poorly explored. We studied the effect of knockdown of Argonaute genes (AGO1, AGO2, AGO3, piwi) in various tissues on the Drosophila melanogaster lifespan and survival after the γ-irradiation at a dose of 700 Gy. In most cases, these parameters were reduced or did not change significantly in flies with tissue-specific RNA interference. Surprisingly, piwi knockdown in both the fat body and the nervous system caused a lifespan increase. But changes in radioresistance depended on the tissue in which the gene was knocked out. In addition, analysis of changes in retrotransposon levels and expression of stress response genes allowed us to determine associated molecular mechanisms. |
| Databáze: | OpenAIRE |
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