Nepetin, a natural compound from Inulae flos, suppresses degranulation and eicosanoid generation through PLCγ1 and Akt signaling pathways in mast cells
| Autor: | Sun-Gun Kim, Hyo-Hyun Park, Eujin Lee, Youn Ju Lee, Meihua Jin, Ning Ji, Eunkyung Lee |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cell Survival Pharmacology Immunoglobulin E medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound Mice Structure-Activity Relationship 0302 clinical medicine Drug Discovery medicine Animals Mast Cells Protein kinase B Biological Products Mice Inbred BALB C biology Leukotriene C4 Dose-Response Relationship Drug Molecular Structure Phospholipase C gamma Prostaglandin D2 Organic Chemistry Anti-Inflammatory Agents Non-Steroidal Degranulation Flavones 030104 developmental biology chemistry Eicosanoid 030220 oncology & carcinogenesis Allergic response biology.protein Molecular Medicine Inula Proto-Oncogene Proteins c-akt Nepetin Signal Transduction |
| Zdroj: | Archives of pharmacal research. 43(2) |
| ISSN: | 1976-3786 |
| Popis: | Nepetin derived from the flowers of Inula japonica, Inulae flos, has been reported to exert several biological activities, including anti-inflammatory responses. In this study, we evaluated the anti-allergic property of nepetin with its molecular mechanisms in bone marrow-derived mast cells (BMMC) and mice. In this in vitro study, we investigated the inhibitory effects of nepetin on degranulation and generation of leukotriene C4 (LTC4) and prostaglandin D2 (PGD2) in IgE/antigen (Ag)-stimulated BMMC. The effect of nepetin on passive cutaneous anaphylaxis (PCA) reaction was also studied in mice. Nepetin reduced degranulation and LTC4 generation in BMMC. The IgE/Ag-mediated signaling pathway demonstrated that nepetin suppressed intracellular Ca2+ level and activation of PLCγ1 and cPLA2. However, MAPKs were not affected by nepetin in BMMC. In addition, nepetin treatment reduced PGD2 production and suppressed cyclooxygenase-2 protein expression via the inhibition of the Akt and nuclear factor-κB signaling pathways. With respect to the local allergic response in vivo, oral administration of nepetin suppressed mast cell-dependent PCA reaction in a dose-dependent manner. The results of this study suggest that nepetin might have an anti-allergic potential related to mast cell-mediated inflammatory diseases. |
| Databáze: | OpenAIRE |
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