iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy
| Autor: | Kunhua Hu, Hongmei Lu, Shaodong Deng, Minghui Zheng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Disease Proteomics Diabetic nephropathy 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Medicine Molecular Biology Incipient diabetic nephropathy business.industry Research Biomarker General Medicine medicine.disease Blood proteins Blot 030104 developmental biology iTRAQ 030220 oncology & carcinogenesis Cancer research Molecular Medicine Biomarker (medicine) business Gelsolin |
| Zdroj: | Clinical Proteomics |
| ISSN: | 1559-0275 1542-6416 |
| DOI: | 10.1186/s12014-019-9253-1 |
| Popis: | Background Diabetic nephropathy is the most frequent cause of end-stage renal disease worldwide. Identification of biomarkers for diabetic nephropathy for early diagnosis may be the key to avoiding damage from this condition. Methods Proteomic iTRAQ technology was first used to identify differentially expressed plasma proteins in type 2 incipient diabetic nephropathy (IDN) using a Q-Exactive mass spectrometer. Results Compared with controls, 57 proteins (32 upregulated and 25 downregulated proteins) were identified. Furthermore, the gelsolin, collectin-11, PTPRJ, and AKAP-7 proteins were confirmed by Western blots as candidate biomarkers for type 2 IDN through ROC analysis. Conclusions These findings offer a theoretical basis for the early treatment of diabetic nephropathy. |
| Databáze: | OpenAIRE |
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