Cytotoxic effects of quartz and chrysotile asbestos: in vitro interspecies comparison with alveolar macrophages
| Autor: | A Seidel, J Schimmelpfeng |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Biology Toxicology medicine.disease_cause complex mixtures Asbestos Cell Line chemistry.chemical_compound Dogs Species Specificity Chrysotile Acetylglucosaminidase medicine Cytotoxic T cell Animals Humans Viability assay Cytotoxicity Cells Cultured Dose-Response Relationship Drug L-Lactate Dehydrogenase Macrophages technology industry and agriculture Dust Quartz respiratory system Pollution Molecular biology Rats Pulmonary Alveoli Macaca fascicularis medicine.anatomical_structure chemistry Cell culture Trypan blue Cattle Pulmonary alveolus |
| Zdroj: | Journal of toxicology and environmental health. 33(2) |
| ISSN: | 0098-4108 |
| Popis: | Cytotoxic effects of DQ12 quartz and chrysotile asbestos on alveolar macrophages of different animal species were compared in vitro. The type of cell reaction toward the cytotoxic dusts was always the same: a loss of cell viability (trypan blue dye exclusion test) was accompanied by the release of cytoplasmic and lysosomal enzymes. The extent of cellular destruction depended upon the amount of dust applied. In the range of 50-100 micrograms/ml quartz or chrysotile asbestos, species-specific variations were observed in the sensitivity of the cells. At this concentration alveolar macrophages of dogs, monkeys, and human patients were damaged to a greater extent than the cells from rats and cattle. Simultaneous incubation of the cells with quartz and L-alpha-dipalmitoyl lecithin resulted in a reduction of the cytotoxic quartz effect. The extent of the protective effect varied according to the species. In the case of chrysotile asbestos no reduction of the fibers cytotoxicity was observed in the presence of L-alpha-dipalmitoyl lecithin. |
| Databáze: | OpenAIRE |
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