GDF9-β promotes folliculogenesis in sheep ovarian transplantation onto the chick embryo chorioallantoic membrane (CAM) in cryopreservation programs
| Autor: | Bryan Woodward, Nahid Yari, Mehdi Mohsenzadeh, Mahboubeh Vatanparast, Mohammad Ali Khalili |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system Growth Differentiation Factor 9 Ovary Chick Embryo Cryopreservation Chorioallantoic Membrane Andrology 03 medical and health sciences 0302 clinical medicine Ovarian Follicle Follicular phase Freezing medicine Animals Humans Fertility preservation Ovarian follicle 030219 obstetrics & reproductive medicine Sheep business.industry Obstetrics and Gynecology Fertility Preservation General Medicine Vitrification Transplantation Chorioallantoic membrane 030104 developmental biology medicine.anatomical_structure Female Folliculogenesis business |
| Zdroj: | Archives of gynecology and obstetrics. 298(3) |
| ISSN: | 1432-0711 |
| Popis: | Ovarian tissue (OT) cryopreservation is a treatment option for fertility preservation among young cancer patients. However, the procedure may involve a reduction in the GDF9-β expression and a delay in follicular growth after thawing and transplantation. The aim of this study was to evaluate whether supplementation of GDF9-β can compensate the reduction of this factor during the cryopresevation process and promote folliculogenesis after transplantation of thawed sheep ovarian tissue.Sheep OT was cryopreserved using two methods of vitrification and slow freezing. Fresh and thawed OTs were then transplanted onto chick embryo chorioallantoic membrane (CAM) and then divided into two groups based on the addition of GDF9-β to the grafted tissue. After 5 days of culture, both histological and immunohistological (Ki-67) assessments were performed to evaluate follicular structure, development, and proliferation. The fibrotic and necrotic areas were measured using MICROVISIBLE software.Folliculogenesis took place in all culture groups, but was significantly improved only in the +GDF9-β cultured group. Also, better follicular structure was preserved in the aforementioned group (p 0.05). When GDF9-β was supplemented to the culture medium, more neovascularization (p 0.05) and better transplantation (p 0.05) was observed. Furthermore, the areas of fibrosis and necrosis were lower in this group rather than the controls. Follicular proliferative activity was significantly higher only in the slow freezing +GDF9-β cultured group.GDF9-β, as a stimulatory factor, not only promoted the folliculogenesis in the fresh ovarian transplant, but also compensated for its reduction during the cryopreservation process. |
| Databáze: | OpenAIRE |
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