Investigation of carbapenemase and mcr-1 genes in carbapenem-resistant Klebsiella pneumoniae isolates
Autor: | Neslihan Genisel, Tuğcan Başyiğit, Tuba Dal, Çiğdem Arabacı, Rıza Durmaz |
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Přispěvatelé: | Dicle Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bililmleri Bölümü, Genişel, Neslihan |
Rok vydání: | 2018 |
Předmět: |
Adult
Ertapenem Male Carbapenem Turkey Klebsiella pneumoniae Cefepime Resistance Tigecycline Microbial Sensitivity Tests Biology Microbiology Polymerase Chain Reaction beta-Lactamases 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Virology medicine Humans 030304 developmental biology Aged Aged 80 and over 0303 health sciences Inpatients 030306 microbiology Colistin General Medicine Middle Aged biology.organism_classification Ethanolaminephosphotransferase Hospitals Klebsiella Infections Infectious Diseases Carbapenem-Resistant Enterobacteriaceae chemistry Amikacin Parasitology MCR-1 Female medicine.drug |
Zdroj: | Journal of infection in developing countries. 13(6) |
ISSN: | 1972-2680 |
Popis: | Introduction: Carbapenem-resistant Klebsiella pneumoniae are a major problem. We aimed to investigate carbapenemase-encoding genes and transferable mcr-1 genes among 57 carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates from hospitalized patients. Methodology: Antibiotic susceptibility tests were performed by Phoenix (BD). Results for ertapenem and colistin were confirmed by gradient diffusion and microdilution methods. Carbapenemase and mcr-1 genes were investigated by Polymerase Chain Reaction (PCR). Results: Thirty-two (56.14%) isolates were from intensive care units (ICU). Antibiotic resistance rates by Phoenix: 52.63% for amikacin; 73.69% trimethoprim sulfamethoxazole; 91.23% cefepime; 82.46% tigecycline; 59.65% colistin. Carbapenemases positivity: 82.45% (47) for blaOXA-48, 40.35% (23) blaOXA-55, 3.50% (2) blaOXA-51, 1.75% (1) blaOXA-23, 1.75% (1) blaOXA-24, 1.75% (1) blaIMP. blaOXA-58, blaKPC, blaNDM-1, and blaVIM were not detected. Twenty (35.08%) isolates had both blaOXA-48 and blaOXA-55. Three isolates were mcr-1 (+) and blaOXA-48 (+). One mcr-1 (+) isolates was blaOXA-51 (+). One colistin sensitive isolate determined by Phoenix, was found colistin resistant by microdilution. Conclusion: OXA-48 and OXA-55 co-harboring isolates and mcr-1 gene (+) isolates were spreading. Automated colistin susceptibility results should be confirmed by microdilution method. Resistance mechanisms in Enterobacteriaceae should be determined and the isolates should be monitored by molecular epidemiological methods. Effective infection control measures will contribute to reduce risk of antibiotic resistant bacterial infections and dissemination of antibiotic resistance. |
Databáze: | OpenAIRE |
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