Over-Expression of a Melon Y3SK2-Type LEA Gene Confers Drought and Salt Tolerance in Transgenic Tobacco Plants
Autor: | Ikuo Nakamura, Peter Nkachukwu Chukwurah, Samuel Aduse Poku, Htut Htet Aung |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine abiotic stress Melon Transgene Plant Science Genetically modified crops Biology 01 natural sciences Article 03 medical and health sciences lcsh:Botany late embryogenesis abundant protein Ecology Evolution Behavior and Systematics Chlorosis Ecology stress tolerance Abiotic stress fungi Wilting food and beverages APX lcsh:QK1-989 Horticulture 030104 developmental biology Germination 010606 plant biology & botany |
Zdroj: | Plants Volume 9 Issue 12 Plants, Vol 9, Iss 1749, p 1749 (2020) |
ISSN: | 2223-7747 |
DOI: | 10.3390/plants9121749 |
Popis: | Climate change, with its attendant negative effects, is expected to hamper agricultural production in the coming years. To counteract these negative effects, breeding of environmentally resilient plants via conventional means and genetic engineering is necessary. Stress defense genes are valuable tools by which this can be achieved. Here we report the successful cloning and functional characterization of a melon Y3SK2-type dehydrin gene, designated as CmLEA-S. We generated CmLEA-S overexpressing transgenic tobacco lines and performed in vitro and in vivo drought and salt stress analyses. Seeds of transgenic tobacco plants grown on 10% polyethylene glycol (PEG) showed significantly higher germination rates relative to wild-type seeds. In the same way, transgenic seeds grown on 150 mM sodium chloride (NaCl) recorded significantly higher germination percentages compared with wild-type plants. The fresh weights and root lengths of young transgenic plants subjected to drought stress were significantly higher than that of wild-type plants. Similarly, the fresh weights and root lengths of transgenic seedlings subjected to salt stress treatments were also significantly higher than wild-type plants. Moreover, transgenic plants subjected to drought and salt stresses in vivo showed fewer signs of wilting and chlorosis, respectively. Biochemical assays revealed that transgenic plants accumulated more proline and less malondialdehyde (MDA) compared with wild-type plants under both drought and salt stress conditions. Finally, the enzymatic activities of ascorbate peroxidase (APX) and catalase (CAT) were enhanced in drought- and salt-stressed transgenic lines. These results suggest that the CmLEA-S gene could be used as a potential candidate gene for crop improvement. |
Databáze: | OpenAIRE |
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