Evaluation of changes in intestinal microbiota in Crohn's disease patients after anti-TNF alpha treatment
| Autor: | Josefa Rodriguez-Morales, Marisa Iborra, Ernesto Cortés-Castell, Juan F. Martinez-Blanch, Jose Maria Paredes-Arquiola, Marina Valls-Gandia, Rafael Gil-Borrás, Maia Boscá-Watts, Laura Sanchis-Artero, Xavier Cortés-Rizo, José María Huguet, Sergio Manresa-Vera |
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| Přispěvatelé: | Producción Científica UCH 2021, UCH. Departamento de Medicina y Cirugía |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Faecalibacterium prausnitzii Diseases Disease THERAPY Gastroenterology 0302 clinical medicine Crohn Disease Young adult Crohn's disease MUCOSA Multidisciplinary biology GUT MICROBIOTA Area under the curve Gastroenterología Middle Aged Treatment Outcome THERAPEUTICS Cohort Medicine Biomarker (medicine) 030211 gastroenterology & hepatology Female Biotechnology Adult medicine.medical_specialty Science Microbiology Article 03 medical and health sciences Young Adult DYSBIOSIS Internal medicine medicine Escherichia coli Humans Crohn's disease - Microbiology Crohn Enfermedad de - Microbiología Gastrointestinal diseases DIGITAL PCR business.industry Aparato digestivo - Enfermedades Case-control study medicine.disease biology.organism_classification Gastrointestinal Microbiome Digestive organs - Diseases FAECALIBACTERIUM-PRAUSNITZII 030104 developmental biology Case-Control Studies FECAL MICROBIOTA Tumor Necrosis Factor Inhibitors business Biomarkers |
| Zdroj: | SCIENTIFIC REPORTS r-FISABIO: Repositorio Institucional de Producción Científica Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO) CEU Repositorio Institucional Fundación Universitaria San Pablo CEU (FUSPCEU) Scientific Reports r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA instname r-FIHGUV. Repositorio Institucional de Producción Científica de la Fundación de Investigación del Hospital General de Valencia r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) r-FISABIO. Repositorio Institucional de Producción Científica |
| ISSN: | 2045-2322 |
| Popis: | Este artículo se encuentra disponible en la siguiente URL: https://www.nature.com/articles/s41598-021-88823-2.pdf En este artículo también participan: Jose Maria Paredes-Arquiola, Maia Boscá-Watts, Jose Maria Huguet, Rafael Gil-Borrás y Josefa Rodríguez-Morales. Intestinal dysbiosis is key in the onset and development of Crohn’s disease (CD). We evaluated the microbiota changes in CD patients before and after a six-month anti-TNF treatment, comparing these changes with the microbiota of healthy subjects. This prospective multicenter observational study involved 27 CD patients initiating anti-TNF treatment and 16 healthy individuals. Inflammatory activity was determined at baseline, 3 and 6 months, classifying patients into responders and nonresponders. Fecal microbiota was analyzed by massive genomic sequencing thought 16S rRNA amplicon sequencing before and after six months of anti-TNF treatment. The CD cohort showed a decrease in genera of the class Clostridia, short-chain fatty acid producers, and an increase in the phylum Proteobacteria (p < 0.01) versus the healthy cohort. After anti-TNF treatment, the phylum Proteobacteria also increased in non-responders versus responders (13/27) (p < 0.005), with the class Clostridia increasing. In addition, alpha diversity increased in responders versus non-responders (p < 0.01), tending towards eubiosis. An association was found (p < 0.001) in the F.prausnitzii/E.coli ratio between responders and non-responders. The F/E ratio was the most accurate biomarker of anti-TNF response (area under the curve 0.87). Thus, anti-TNF treatment allows partial restoration of intestinal microbiota in responders and the F.prausnitzii/E.coli ratio can provide a reliable indicator of response to anti-TNF in CD. |
| Databáze: | OpenAIRE |
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