A polynuclear Cu(ii) complex for real time monitoring of mitochondrial cytochrome C release during cellular apoptosis
| Autor: | Paula Brandão, Milan Ghosh, Subhasis Ghosh, Ankush Paladhi, Somnath Khanra, Sumit Kumar Hira, Vítor Félix, Sabyasachi Ta, Partha Partim Manna, Debasis Das |
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| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Chromosomal translocation Apoptosis 02 engineering and technology 010402 general chemistry 01 natural sciences Catalysis Cell Line Mice Coordination Complexes Materials Chemistry Animals Humans biology Chemistry Cytochrome c Metals and Alloys Cytochromes c General Chemistry 021001 nanoscience & nanotechnology Binding constant Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Mitochondria Hydrazines Microscopy Fluorescence Cell culture Spectrophotometry Ceramics and Composites Biophysics biology.protein 0210 nano-technology Copper Conjugate |
| Zdroj: | Chemical communications (Cambridge, England). 56(48) |
| ISSN: | 1364-548X |
| Popis: | A new amide-imine conjugate, 2-hydroxybenzoic acid-(2-hydroxybenzylidene)-hydrazide (L1), is employed to prepare a single crystal X-ray structurally characterized poly-nuclear Cu(ii) complex (M1). M1 selectively and spatially interacts with cytochrome C (Cyt C) to allow fluorescence imaging of intracellular translocation events in living cells. Thus, direct visualization of a Cyt C translocation event during an apoptotic process is achieved for the first time. The binding constant and LOD are 7.52 × 104 M-1 and 34.0 nM, respectively. |
| Databáze: | OpenAIRE |
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