ω-3 Polyunsaturated fatty acids accelerate airway repair by activating FFA4 in club cells
| Autor: | Saeromi Kang, Fumikazu Okajima, Soo-Jin Park, Koichi Sato, Hae Young Chung, Dong-Soon Im, Kyoung-Pil Lee, Jung-Min Koh |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pulmonary and Respiratory Medicine medicine.medical_specialty Bronchiole Docosahexaenoic Acids Physiology Cellular differentiation Cell Separation Naphthalenes Biology Receptors G-Protein-Coupled 03 medical and health sciences 0302 clinical medicine Cell Movement Physiology (medical) Internal medicine Fatty Acids Omega-3 medicine Animals Receptor Lung Cell Proliferation Mice Knockout chemistry.chemical_classification Mice Inbred BALB C Wound Healing Cell growth GPR120 Cell Differentiation Lung Injury Cell Biology Drug Combinations 030104 developmental biology medicine.anatomical_structure Club cell Endocrinology Eicosapentaenoic Acid 030228 respiratory system chemistry Docosahexaenoic acid Immunology Polyunsaturated fatty acid |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 312:L835-L844 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00350.2016 |
| Popis: | A G protein-coupled receptor (GPCR) named free fatty acid receptor 4 (FFA4, also known as GPR120) was found to act as a GPCR for ω-3 polyunsaturated fatty acids. Its expression has been reported in lung epithelial club cells. We investigated whether supplementation of the ω-3 fatty acids benefits lung health. Omacor (7.75 mg/kg), clinically prescribed preparation of ω-3 fatty acids, and FFA4-knockout mice were utilized in a naphthalene-induced mouse model of acute airway injury (1 injection of 30 mg/kg ip). Naphthalene injection induced complete destruction of bronchiolar epithelial cells within a day. Appearance of bronchiolar epithelial cells was observed after 21 days in control mice. It was found, however, that supplementation of Omacor accelerated the recovery. The appearance of bronchiolar epithelial cells was observed between 7 and 14 days after naphthalene injury in Omacor-treated mice. In isolated club cells, ω-3 fatty acids were found to stimulate cell proliferation and migration but to inhibit cell differentiation. With the use of pharmacological tools and FFA4-knockout mice, FFA4 was found to be responsible for ω-3 fatty acids-induced proliferation in vitro in club cells. Furthermore, accelerated recovery from naphthalene-induced airway injury in Omacor-treated mice was not observed in FFA4-knockout mice in vivo. Present findings indicate that ω-3 fatty acids-induced proliferation of bronchiole epithelial cells through FFA4 is responsible for Omacor-induced accelerated recovery from airway injury. Therefore, intermittent administration of Omacor needs to be tested for acute airway injury because ω-3 fatty acids stimulate proliferation but inhibit differentiation of club cells. |
| Databáze: | OpenAIRE |
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