XIST knockdown suppresses vascular smooth muscle cell proliferation and induces apoptosis by regulating miR-1264/WNT5A/β-catenin signaling in aneurysm
| Autor: | Peng-Fei Xia, Liang Zou, Yan-Yan Hou, Lei Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Vascular smooth muscle proliferation Myocytes Smooth Muscle Biophysics Apoptosis Biochemistry Muscle Smooth Vascular Wnt-5a Protein 03 medical and health sciences Mice 0302 clinical medicine abdominal aortic aneurysm lncRNA Animals Humans Molecular Biology Wnt Signaling Pathway Cells Cultured beta Catenin Research Articles Aged Cell Proliferation Cancer Gene knockdown miR-1264 Chemistry Competing endogenous RNA Wnt signaling pathway Cell Biology Middle Aged Cell biology WNT5A Mice Inbred C57BL MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis cardiovascular system XIST Female RNA Long Noncoding Epigenetics Signal transduction Aortic Aneurysm Abdominal |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 |
| Popis: | Long non-coding RNAs (lncRNAs) have been ascertained as vital modulators in abdominal aortic aneurysm (AAA) development. In this research, the function and molecular mechanisms of the lncRNA X-inactive specific transcript (XIST) in the evolution of vascular smooth muscle cells (VSMCs) were assessed. Results showed that XIST expression was increased but miR-1264 expression level was reduced in the serum of AAA patients. XIST depletion impeded human aorta VSMCs (HA-VSMCs’) ability to proliferate and stimulate apoptosis, while repressing miR-1264 expression through an unmediated interaction. Additionally, the influence of XIST knockdown on apoptosis and proliferation could be rescued by an miR-1264 inhibitor. Subsequent molecular investigations indicated that WNT5A was miR-1264’s target, and XIST functioned as a competing endogenous RNA (ceRNA) of miR-1264 to raise WNT5A expression. Further, an miR-1264 inhibitor stimulated the proliferation and suppressed the apoptosis of HA-VSMCs through the activation of WNT/β-catenin signaling. Taken together, XIST impeded the apoptosis and stimulated the proliferation of HA-VSMCs via the WNT/β-catenin signaling pathway through miR-1264, demonstrating XIST’s underlying role in AAA. |
| Databáze: | OpenAIRE |
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