Tumor spheroid assembly on hyaluronic acid-based structures: A review
| Autor: | Sónia P. Miguel, Ilídio J. Correia, Elisabete C. Costa, Marco António Paulo de Carvalho |
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| Přispěvatelé: | uBibliorum |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Polymers and Plastics Hyaluronic acid Nanotechnology 02 engineering and technology Tumor spheroid 03 medical and health sciences chemistry.chemical_compound Spheroids Cellular Tumor Cells Cultured Tumor Microenvironment Materials Chemistry medicine Animals Humans HA-based structure CD44 biology Cell growth Organic Chemistry Spheroid Cancer 021001 nanoscience & nanotechnology medicine.disease In vitro Cell biology 030104 developmental biology chemistry Cell culture Cancer cell biology.protein Tumor extracellular matrix 0210 nano-technology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP |
| Popis: | Two-dimensional (2D) cell culture is the main methodology used for screening anticancer therapeutics. However, these 2D cellular models misrepresent the architecture of native tumors, leading, in some cases, to unsuccessful prediction of cancer cell response to drugs. To overcome such limitations, cell growth in three dimensions (3D) arises as an alternative to reproduce in vitro the cellular arrangement found in tumors. Among the 3D cancer models developed so far, spheroids are the most attractive since these are cellular aggregates that broadly mimic many features of solid tumors affecting humans, like cell-cell interactions. One of the most applied techniques for producing spheroids is the liquid overlay technique, in which cells aggregate due to their limited adhesion to certain biomaterials, usually agarose or agar. Recently, the suitability of hyaluronic acid (HA) for spheroids assembly and HA-cell surface receptor interactions has been investigated. Ergo, this review gathers a summary of different studies where HA-based structures were developed and used for tumor spheroids production in order to be used in vitro as reliable 3D tumor models for therapeutic screening purposes. |
| Databáze: | OpenAIRE |
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