Sensitive genotyping of mutations in the EGFR gene from NSCLC patients using PCR-GoldMag lateral flow device
| Autor: | Qian Liu, Mingwei Chen, Juanli Zhu, Chao Zhang, Yali Cui, Qinlu Zhang, Xue-Min Yang, Wenli Hui, Xian-Ying Li |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lung Neoplasms Genotype Science Biology medicine.disease_cause Polymerase Chain Reaction Article 03 medical and health sciences Exon 0302 clinical medicine Carcinoma Non-Small-Cell Lung Carcinoma medicine Humans Prospective Studies Epidermal growth factor receptor Genotyping Gene Mutation Multidisciplinary Case-control study medicine.disease Molecular biology respiratory tract diseases ErbB Receptors 030104 developmental biology Case-Control Studies 030220 oncology & carcinogenesis biology.protein Medicine |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-08210-8 |
| Popis: | Epidermal growth factor receptor (EGFR) mutations predict better outcomes with EGFR tyrosine kinase inhibitors in patients with non-small cell lung cancer (NSCLC). Most common activating mutations include in-frame deletion in exon 19 and L858R substitution in exon 21, which account for >90% of all EGFR mutations in NSCLC. In this study, a PCR-GoldMag lateral flow assay (PCR-GoldMag LFA) was developed for the visual detection of delE746-A750 and L858R of EGFR mutations. Forty formalin-fixed paraffin-embedded (FFPE) tissue samples of NSCLC patients were analyzed using PCR-GoldMag LFA system and verified by direct sequencing and TaqMan-PCR detection methods. Results showed that EGFR mutations were detected in 34 cases among the 40 samples (85%) by PCR-GoldMag LFA method. Among the 34 cases, 5 cases were simultaneously detected with delE746-A750 in exon 19 and L858R mutation in exon 21. Compared with sequencing, only 4 samples were detected as delE746-A750, which revealed higher sensitivity of PCR-GoldMag LFA detection method than direct sequencing. TaqMan-PCR method verified the L858R mutation and was in 100% agreement with our method. These results indicated that our method has obvious advantages to analyze clinical samples and offers a more sensitive alternative to direct sequencing for the detection of EGFR mutations. |
| Databáze: | OpenAIRE |
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