Evaluation of two interspecific recombinant viruses generated from two neurotropic bovine alphaherpesviruses: genomic characterization and virulence properties in the natural host
| Autor: | Maria Paula Del Medico, Etienne Thiry, François Meurens, Maria Fatima Ladelfa, Sonia Alejandra Romera, Benoît Muylkens, Fiorella Kotsias, Günther M. Keil, Julien Thiry |
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| Přispěvatelé: | Inconnu, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Base pair
[SDV]Life Sciences [q-bio] lcsh:Medicine Virulence Biology Genome General Biochemistry Genetics and Molecular Biology Homology (biology) law.invention 03 medical and health sciences law lcsh:Science Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences 030306 microbiology lcsh:R General Medicine biology.organism_classification Virology Bovine herpesvirus 1 3. Good health [SDV] Life Sciences [q-bio] Bovine herpesvirus 5 Recombinant DNA lcsh:Q |
| Zdroj: | Infectious diseases of the nervous system: pathogenesis and worlwide impact. Infectious diseases of the nervous system: pathogenesis and worlwide impact., 2008, Paris, France. 2 p., 2008 BMC Proceedings, Vol 2, Iss Suppl 1, p P14 (2008) |
| Popis: | Bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5(BoHV-5) are two closely related alphaherpesviruses thatinfect cattle. Both viruses are neurotropic but only BoHV-5 significantly replicates in the central nervous system andinduces neurological disease. We previously isolated 2interspecific recombinant viruses (R1 and R2) betweenBoHV-1 and BoHV-5 [1]. We report here on the precisecharacterization of the genetic backgrounds and on thevirulence assessment of these two BoHV-1/5 recom-binants in their natural host.An exhaustive PCR/sequencing approach led us to iden-tify the precise location of the two cross-over (CO) pointswhere recombination events occurred between the paren-tal strains. The first CO occurred in a 44 base pairs (bp)sequence of homology at the 3' end of the UL28 encodinggenes of BoHV-1 and BoHV-5. The resulting recombinant(R1) inherited 37% of the BoHV-1 genome. The rest of itsgenome (63%) is homologous to BoHV-5 containing thefinal region of the unique long (UL) region (from UL27 toUL0.5), the internal and terminal repeats (IR and TR) andthe unique short region (US). The second CO occurred ina 39 bp sequence of homology within the UL43 gene. Theresulting recombinant (R2) is mainly composed of BoHV-1 sequences (86%). It is homologous to BoHV-1 in a largeportion of the UL region (from UL43 to UL0.5), in the IR/TR and in the US region. BoHV-5 sequences account for14% of its genome content.In order to study the |
| Databáze: | OpenAIRE |
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