Isolation and Expression Analysis of Petunia CURLY LEAF-Like Genes
| Autor: | Suguru Tsuchimoto, Tomoko Mayama, Eiichi Ohtsubo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Physiology
Sequence analysis Molecular Sequence Data Plant Science Biology Genes Plant Homology (biology) Gene Expression Regulation Plant Amino Acid Sequence RNA Messenger Enhancer Gene Conserved Sequence Plant Proteins Homeodomain Proteins Genetics Regulation of gene expression Sequence Homology Amino Acid Agamous Gene Expression Profiling Cell Biology General Medicine Protein Structure Tertiary Petunia Gene expression profiling Alternative Splicing RNA Plant Mutation Homeotic gene |
| Zdroj: | Plant and Cell Physiology. 44:811-819 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcg102 |
| Popis: | The Arabidopsis CURLY LEAF (CLF) gene is required to repress transcription of the class C gene AGAMOUS (AG) in whorls 1 and 2 of flowers and also in vegetative organs. CLF encodes a protein with homology to the product of the Drosophila Polycomb-group gene Enhancer of zeste [E(z)], which is involved in embyogenesis. In this study, we isolated three petunia CLF-like genes (PhCLF1, PhCLF2 and PhCLF3) based on the sequence homology between CLF and E(Z). Sequence analysis suggests that PhCLF1 and PhCLF2 are orthologs of CLF, whereas PhCLF3 is an ortholog of the Arabidopsis gene EZA1. We identified several conserved domains among products of PhCLF genes and related genes. PhCLF1 and PhCLF2 were expressed in all floral organs and leaves. The PhCLF1 transcripts were accumulated especially in corolla limbs, and contained several alternatively spliced RNA species. PhCLF1 and PhCLF2 do not appear to be the BLIND gene, which is required to repress transcription of the petunia class C gene, but their expression was affected by the homeotic conversion of organs in the blind flower. Our findings show that expression of PhCLF1 is regulated differently from that of PhCLF2, and suggest that the two petunia CLF orthologs function differently from each other. |
| Databáze: | OpenAIRE |
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