Perfluorotridecanoic Acid Inhibits Leydig Cell Maturation in Male Rats in Late Puberty via Changing Testicular Lipid Component
| Autor: | Ying Zhong, Yang Li, Haoni Yan, Dapang Rao, Cheng Zou, Zina Wen, Xiaoheng Li, Ren-Shan Ge, Changchang Li, Yiyan Wang, Peipei Pan, Yingfen Ying, Huitao Li, Song Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Linoleic acid Administration Oral 010501 environmental sciences Toxicology 01 natural sciences Rats Sprague-Dawley Palmitic acid 03 medical and health sciences chemistry.chemical_compound Internal medicine Testis medicine Animals 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification Fluorocarbons 0303 health sciences Dose-Response Relationship Drug Triglyceride Leydig cell Leydig Cells Fatty acid General Medicine Epididymis Lipids Rats Oleic acid medicine.anatomical_structure Endocrinology chemistry Pituitary Gland Decanoic Acids Hormone |
| Zdroj: | Chemical Research in Toxicology. 34:1542-1555 |
| ISSN: | 1520-5010 0893-228X |
| DOI: | 10.1021/acs.chemrestox.0c00458 |
| Popis: | Perfluorotridecanoic acid (PFTrDA) is a long-chain (C13) perfluoroalkyl carboxylic acid. Here, we report the influence of PFTrDA exposure on the maturation of rat Leydig cells in late puberty in vivo. Male Sprague-Dawley rats were administered PFTrDA by gavage of 0, 1, 5, and 10 mg/kg/day from 35 days to 56 days postpartum. PFTrDA had no effect on body weight, testis weight, and epididymis weight. It significantly decreased the serum testosterone level after 5 and 10 mg/kg exposure, while it did not alter the serum estradiol level. The serum luteinizing hormone level was markedly reduced after 10 mg/kg PFTrDA exposure, while the follicle-stimulating hormone level was unchanged. Star, Cyp11a1, Cyp17a1, Hsd3b1, and Insl3 transcript levels in the testis were markedly lowered in the 1-5 mg/kg PFTrDA group and the Lhb transcript level in the pituitary in the 10 mg/kg group. CYP11A1 and HSD11B1-positive Leydig cell numbers were markedly reduced after 10 mg/kg PFTrDA exposure. Testicular triglyceride and free fatty acid (palmitic acid, oleic acid, and linoleic acid) levels were significantly reduced by PFTrDA, while Mgll (up-regulation) and Scarb1 and Elovl5 (down-regulation) expression were altered. AKT1 and AMPK phosphorylation was stimulated after 10 PFTrDA mg/kg exposure. In conclusion, PFTrDA delays the maturation of Leydig cells in late puberty mainly by altering the free fatty acid profile. |
| Databáze: | OpenAIRE |
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