Cd(II), Pb(II) and Zn(II) ions regulate expression of the metal-transporting P-type ATPase ZntA inEscherichia coli
| Autor: | Robert K. Poole, Marie R.B. Binet |
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| Rok vydání: | 2000 |
| Předmět: |
Cations
Divalent ATPase Gene Dosage Biophysics Microbial Sensitivity Tests medicine.disease_cause Biochemistry Substrate Specificity Metal Adenosine Triphosphate Bacterial Proteins Structural Biology ATP hydrolysis Operon P-type ATPase ZntA Escherichia coli Genetics Transcriptional regulation medicine Transcriptional regulator ZntR Inducer Promoter Regions Genetic Molecular Biology Adenosine Triphosphatases Regulation of gene expression biology Chemistry Escherichia coli Proteins Hydrolysis Drug Resistance Microbial Gene Expression Regulation Bacterial Cell Biology Cd(II) Molecular biology Up-Regulation Pb(II) Zinc Zn(II) Lead Genes Bacterial visual_art Mutation visual_art.visual_art_medium biology.protein P-type ATPase Cadmium Transcription Factors |
| Zdroj: | FEBS Letters. 473:67-70 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(00)01509-x |
| Popis: | ZntA is a cation-translocating ATPase which exports from Escherichia coli Cd(II) and Pb(II), as well as Zn(II). The metal-dependent ATP hydrolysis activity of purified ZntA was recently characterised and showed a specificity for Cd(II), Pb(II) and Zn(II). zntA expression has been reported to be up-regulated primarily by Zn(II), mediated by the regulatory protein ZntR, belonging to the MerR transcriptional regulator family. In contrast to previous claims, we now show, using a Φ(zntA-lacZ) monolysogen, that Cd(II) is the most effective inducer of zntA, which is also induced significantly by Pb(II). The Cd(II)- and Pb(II)-dependent transcriptional up-regulation of zntA is also mediated by ZntR. |
| Databáze: | OpenAIRE |
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