HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms
| Autor: | Shrestha Binita, Pornpen Dararat, Satoshi Gando, Kiyoshi Kikuchi, Yuko Nawa, Tomoka Nagasato, Mika Yamamoto, Ikuro Maruyama, Salunya Tancharoen, Somphong Narkpinit |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Keratinocytes Palate Hard Vascular Endothelial Growth Factor A Pathology medicine.medical_treatment palatal mucosa Receptor for Advanced Glycation End Products Gingiva NF-κB RAGE (receptor) Tissue Culture Techniques Mice Maxilla HMGB1 Protein RNA Small Interfering Spectroscopy HMGB1 Mice Knockout biology Chemistry Antibodies Monoclonal Cell migration General Medicine Immunohistochemistry Computer Science Applications medicine.anatomical_structure Signal Transduction medicine.medical_specialty Connective tissue chemical and pharmacologic phenomena Catalysis Article Inorganic Chemistry 03 medical and health sciences Proliferating Cell Nuclear Antigen medicine wound healing Animals Physical and Theoretical Chemistry Molecular Biology Wound Healing Growth factor Organic Chemistry Mouth Mucosa NF-kappa B p50 Subunit Molecular biology Epithelium Proliferating cell nuclear antigen 030104 developmental biology Gene Expression Regulation Cell culture biology.protein Wound healing |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 17; Issue 11; Pages: 1961 |
| ISSN: | 1422-0067 |
| Popis: | High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 (Hmgb1+/−) mice and Wild-type (WT) mice were subjected to palatal injury. Maxillary tissues were stained with Mallory Azan or immunostained with anti-HMGB1, anti-proliferating cell nuclear antigen (PCNA), anti-nuclear factor-κB (NF-κB) p50 and anti-vascular endothelial growth factor (VEGF) antibodies. Palatal gingival explants were cultured with recombinant HMGB1 (rHMGB1) co-treated with siRNA targeting receptor for advanced glycation end products (RAGEs) for cell migration and PCNA expression analysis. Measurement of the wound area showed differences between Hmgb1+/− and WT mice on Day 3 after wounding. Mallory Azan staining showed densely packed of collagen fibers in WT mice, whereas in Hmgb1+/− mice weave-like pattern of low density collagen bundles were present. At three and seven days post-surgery, PCNA, NF-κB p50 and VEGF positive keratinocytes of WT mice were greater than that of Hmgb1+/− mice. Knockdown of RAGE prevents the effect of rHMGB1-induced cell migration and PCNA expression in gingival cell cultures. The data suggest that HMGB1/RAGE axis has crucial roles in palatal wound healing. |
| Databáze: | OpenAIRE |
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