Evidence of mitochondrial involvement in scrapie infection
| Autor: | J L Williamson, Richard F. Marsh, Judd M. Aiken |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Male
Mitochondrial DNA PrPSc Proteins Blotting Western Immunology Hamster Scrapie Mitochondrion Biology Cell Fractionation Microbiology Viral Proteins chemistry.chemical_compound Cricetinae Virology Complementary DNA Animals Electrophoresis Gel Two-Dimensional Cloning Molecular Cytoskeleton Infectivity Cell Membrane Brain DNA Molecular biology Mitochondria Proton-Translocating ATPases Digitonin chemistry Insect Science Nucleic acid Research Article |
| Zdroj: | Journal of Virology. 63:1686-1694 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.63.4.1686-1694.1989 |
| Popis: | Two cDNA libraries were constructed from brain membrane and cytoskeletal preparations purified from scrapie-infected hamster brains. Four recombinants strongly preferential to the scrapie cytoskeletal preparation were identified by the differential hybridization of 7,000 recombinants. These clones were not, however, preferential to total nucleic acids extracted from scrapie-infected hamster brains. DNA sequence analysis revealed all four clones to have significant sequence similarities to the mouse mitochondrial genome. This correlation led us to consider a mitochondrial association with scrapie infectivity. Brain mitochondria were purified by sucrose gradient density centrifugation and found to contain high infectivity. Removal of mitochondrial outer membranes by osmotic shock or digitonin treatment resulted in no detectable loss of titer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|