Immunization with Toscana virus N-Gc proteins protects mice against virus challenge
| Autor: | Gianni Gori Savellini, Colomba Giorgi, Giuseppa Di Genova, Maria Grazia Cusi, Jean Denis Docquier, Paola Di Bonito, Melissa Valentini, Chiara Terrosi |
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| Rok vydání: | 2007 |
| Předmět: |
Envelope glycoprotein
CD8-Positive T-Lymphocytes Antibodies Viral Virus Interferon-gamma Mice Immune system Antigen Viral Envelope Proteins Neutralization Tests Virology medicine Animals Humans Cells Cultured Immunization Schedule Toscana virus Nucleocapsid protein Mice Inbred BALB C Vaccines Synthetic biology Meningoencephalitis Sandfly fever Naples virus Viral Vaccines Nucleocapsid Proteins biology.organism_classification medicine.disease Cytotoxicity Tests Immunologic Phlebotomus Fever Immunization biology.protein Female Antibody Vaccine Encephalitis Injections Intraperitoneal Spleen |
| Zdroj: | Virology. 375(2) |
| ISSN: | 0042-6822 |
| Popis: | Toscana virus (TOSV) is an emerging virus, circulating in the Mediterranean area, that is responsible for aseptic meningitis, meningoencephalitis, and encephalitis. The development of a vaccine that could provide complete protection from TOSV infection is needed. In this study we investigated the capacity of TOSV structural proteins, nucleocapsid protein N and the two Gc and Gn glycoproteins, produced as recombinant proteins, in an animal model. In particular, we investigated their role in inducing specific and protective immune responses against virus infection. Mice were immunized intraperitoneally using TOSV antigens singly or in combination. The results show that only the N-Gc combination was able to protect 100% of animals from a lethal challenge with a neurovirulent strain of TOSV. This potential vaccine induces high serum antibody titres with neutralizing activity and it is safe for animals. Moreover, immunization induces a virus specific cell-mediated immune response, in particular a CD8+ T cell response associated with a marked expression of interferon gamma. These results indicate that the N+Gc viral antigen combination could be useful for future development of a vaccine controlling the spread of this emerging virus that could pose a new threat for humans. |
| Databáze: | OpenAIRE |
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