Isolation and functional analysis of the CjNdly gene, a homolog in Cryptomeria japonica of FLORICAULA/LEAFY genes
| Autor: | Morohoshi Noriyuki, Daisuke Murasugi, Kosuke Osanai, Kenji Shinohara, Yoshihiro Katayama, Shinya Yamada, Shinya Kawai, Shinya Kajita, Norihiro Futamura, Takahiro Shiokawa |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Plant Physiology Cryptomeria Molecular Sequence Data Plant Science Genes Plant Japonica Homology (biology) Botany Amino Acid Sequence Gene Peptide sequence Leafy 3' Untranslated Regions Conserved Sequence Phylogeny DNA Primers Plant Proteins Genetics biology fungi Nucleic acid sequence food and beverages biology.organism_classification Plant Leaves RNA Plant Heterologous expression 5' Untranslated Regions |
| Zdroj: | Tree physiology. 28(1) |
| ISSN: | 0829-318X |
| Popis: | We report the isolation and characterization of CjNdly, a homolog in Japanese cedar (Cryptomeria japonica D. Don) of the FLORICAULA/LEAFY (FLO/LFY) genes. We determined the entire nucleotide sequence of CjNdly, including short 5'- and 3'-untranslated regions. The deduced amino acid sequence was similar to those of the products of the FLO/LFY genes from other species. The nucleotide sequence showed the closest homology to that of the NEEDLY gene in Pinus radiata D. Don. Although no proline-rich region has been reported previously in homologous gene products from gymnosperms, we found such a region at the amino-terminal end of the deduced amino acid sequence encoded by CjNdly. We detected the expression of CjNdly in both reproductive and vegetative tissues and organs of C. japonica. Heterologous expression of CjNdly in transgenic tobacco plants induced precocious flowering of regenerating shoots on agar-solidified medium and flowers with an abnormal phenotype, namely, petal-like stamens. Our findings suggest that the CjNdly gene may have important roles in flower development in Japanese cedar, resembling those of its angiosperm homologs. |
| Databáze: | OpenAIRE |
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