Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations
| Autor: | Lucas Domínguez, Inmaculada Moreno, Álvaro Roy, Christian Gortázar, Javier Bezos, Eugenia Puentes, J. Åkerstedt, María Ángeles Risalde, Beatriz Romero, G. T. Tessema, José Antonio Infantes-Lorenzo, Mercedes Domínguez, Ana Balseiro, L. de Juan |
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| Přispěvatelé: | Ministerio de Agricultura, Pesca y Alimentación (España), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España), Ministerio de Economía y Competitividad (España), Ministerio de Economía, Industria y Competitividad (España), European Commission, CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ministerio de Educación, Cultura y Deporte (España), Ministerio de Ciencia, Innovación y Universidades (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Veterinary medicine
Tuberculosis P22 040301 veterinary sciences Swine Corynebacterium pseudotuberculosis Paratuberculosis Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Serology Animal Diseases 0403 veterinary science 03 medical and health sciences Blood serum Seroepidemiologic Studies medicine Animals Animal tuberculosis 030304 developmental biology 0303 health sciences Pig lcsh:Veterinary medicine Sheep General Veterinary biology Norway Goats 04 agricultural and veterinary sciences General Medicine Mycobacterium tuberculosis biology.organism_classification medicine.disease Vaccination Mycobacterium avium subsp. paratuberculosis Mycobacterium tuberculosis complex Spain Herd Goat lcsh:SF600-1100 Cattle Serum antibodies Research Article |
| Zdroj: | BMC Veterinary Research Repisalud Instituto de Salud Carlos III (ISCIII) Digital.CSIC. Repositorio Institucional del CSIC instname BMC Veterinary Research, Vol 15, Iss 1, Pp 1-8 (2019) |
| ISSN: | 1746-6148 |
| Popis: | [Background]: Serum antibody detection has potential as a complementary diagnostic tool in animal tuberculosis (TB) control, particularly in multi-host systems. The objective of the present study was to assess the specificity (Sp) of an enzyme-linked immunosorbent assay (ELISA) based on the new multiprotein complex P22 for the detection of specific antibodies against the Mycobacterium tuberculosis complex (MTC) in the four most relevant domestic animals acting as MTC hosts: cattle, goat, sheep and pig. We used sera from an officially TB-free (OTF) country, Norway, and from a non-OTF one, Spain. The samples included sera from goats that had been vaccinated against M. avium subsp. paratuberculosis (MAP) and sheep from a herd in which Corynebacterium pseudotuberculosis had been isolated. [Results]: In cattle, the Sp ranged from 92.5 (IC95% 90.7–94) to 99.4% (IC95% 98.3–99.8) depending on the cut-off used and the origin of the samples (Spain or Norway). Sp in cattle (cut-off point 100) was significantly higher (P [Conclusions]: Due to its excellent Sp in pigs and acceptable Sp in cattle and sheep, this ELISA may constitute a suitable option for TB screening at herd level, particularly in OTF-countries. Jose Antonio Infantes-Lorenzo was supported by an FPU contract-fellowship (Formación de Profesorado Universitario) from the Ministerio de Educación, Cultura y Deporte of the Spanish Government (FPU2013/6000). Alvaro Roy is the recipient of an Industrial Doctorate contract (DI-15-08110) funded by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and the European Social Fund. Javier Bezos is recipient of a Juan de la Cierva Incorporación research contract (IJCI-2015-24805) funded by the MINECO. This work was supported by the Spanish Ministry of Agriculture, Fisheries and Food and the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria of Spain (RTA2015–00043-C02–02). This is a contribution to Plan Nacional de I + D + i grant CGL2017–89866 WILD-DRIVER. |
| Databáze: | OpenAIRE |
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