Biotransformation of insecticide flonicamid by Aminobacter sp. CGMCC 1.17253 via nitrile hydratase catalysed hydration pathway
| Autor: | Zhi-Ling Dai, Xi Cheng, Yi-Jun Dai, Zhi-Xia Fan, Huo-Yong Jiang, Wen-Long Yang |
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| Rok vydání: | 2020 |
| Předmět: |
Niacinamide
Insecticides Aminobacter sp Stereochemistry Applied Microbiology and Biotechnology 03 medical and health sciences Bioremediation Bacterial Proteins Biotransformation Genus Aminobacter Nitrile hydratase Soil Pollutants Hydro-Lyases 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 030306 microbiology Chemistry Phyllobacteriaceae General Medicine biology.organism_classification Recombinant Proteins Transformation (genetics) Biodegradation Environmental Enzyme Bacteria Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 130:1571-1581 |
| ISSN: | 1365-2672 1364-5072 |
| Popis: | AIMS This study evaluates flonicamid biotransformation ability of Aminobacter sp. CGMCC 1.17253 and the enzyme catalytic mechanism involved. METHODS AND RESULTS Flonicamid transformed by resting cells of Aminobacter sp. CGMCC 1.17253 was carried out. Aminobacter sp. CGMCC 1.17253 converts flonicamid into N-(4-trifluoromethylnicotinoyl) glycinamide (TFNG-AM). Aminobacter sp. CGMCC 1.17253 transforms 31·1% of the flonicamid in a 200 mg l-1 conversion solution in 96 h. Aminobacter sp. CGMCC 1.17253 was inoculated in soil, and 72·1% of flonicamid with a concentration of 0·21 μmol g-1 was transformed in 9 days. The recombinant Escherichia coli expressing Aminobacter sp. CGMCC 1.17253 nitrile hydratase (NHase) and purified NHase were tested for the flonicamid transformation ability, both of them acquired the ability to transform flonicamid into TFNG-AM. CONCLUSIONS Aminobacter sp. CGMCC 1.17253 transforms flonicamid into TFNG-AM via hydration pathway mediated by cobalt-containing NHase. SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report that bacteria of genus Aminobacter has flonicamid-transforming ability. This study enhances our understanding of flonicamid-degrading mechanism. Aminobacter sp. CGMCC 1.17253 has the potential for bioremediation of flonicamid pollution. |
| Databáze: | OpenAIRE |
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