14-3-3-Regulated Ca2+-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis
| Autor: | Daniel Da Silva, Patrice Thuleau, Christian Mazars, Christophe Lachaud, Valérie Cotelle, E Prigent, Sabine Grat, Christian Brière |
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| Rok vydání: | 2012 |
| Předmět: |
Programmed cell death
Arabidopsis Apoptosis Biology Gene Knockout Techniques chemistry.chemical_compound Lanthanum Sphingosine Phosphorylation Molecular Biology Cells Cultured Original Paper Arabidopsis Proteins Kinase Cell Biology Plants Genetically Modified biology.organism_classification Sphingolipid Hedgehog signaling pathway Cell biology 14-3-3 Proteins Biochemistry chemistry Calcium-Calmodulin-Dependent Protein Kinases Second messenger system Calcium Protein Binding |
| Zdroj: | Cell Death & Differentiation. 20:209-217 |
| ISSN: | 1476-5403 1350-9047 |
| DOI: | 10.1038/cdd.2012.114 |
| Popis: | In eukaryotic cells, sphingoid long chain bases (LCBs) such as sphingosine or phytosphingosine (PHS) behave as second messengers involved in various processes including programmed cell death (PCD). In plants, induction of PCD by LCBs has now been described, but the signalling pathway is still enigmatic. Using Arabidopsis, we identify new key steps in this pathway. We demonstrate that PHS induces activation of the calcium-dependent kinase CPK3, which phosphorylates its binding partners, the 14-3-3 proteins. This phosphorylation leads to the disruption of the complex and to CPK3 degradation. Using cpk3 knockout lines, we demonstrate that CPK3 is a positive regulator of LCB-mediated PCD. These findings establish 14-3-3-regulated CPK3 as a key component of the LCB pathway leading to PCD in plants. |
| Databáze: | OpenAIRE |
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