| Autor: |
Albert Bolhuis, K.J. Leenhouts, A. Ledeboer, Gerhardus Venema, Jan Kok |
| Přispěvatelé: |
Groningen Biomolecular Sciences and Biotechnology |
| Jazyk: |
angličtina |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Applied Microbiology and Biotechnology, 44(1). SPRINGER |
| ISSN: |
0175-7598 |
| Popis: |
A plant α-galactosidase gene was inserted in the expression vector pGKV259. The resulting plasmid pGAL2 consisted of the replication functions of the broad-host-range lactococcal plasmid pWV01, the lactococcal promoter P59, and the DNA sequences encoding the α-amylase signal sequence from Bacillus amyloliquefaciens and the mature part of the α-galactosidase from Cyamopsis tetragonoloba (guar). Lactococcus cells of strain MG1363 harbouring this vector produced the plant α-galactosidase and secreted the enzyme efficiently as judged by Western blotting and activity assays. Expression levels of up to 4.3 mg extracellular α-galactosidase g (dry weight) of biomass−1 were achieved in standard laboratory batch cultures. The α-galactosidase produced by Lactococcus was active on the chromogenic substrate 5-bromo-4-chloro-3-indolyl α-d-galactopyranoside, the trisaccharide raffinose and on the galactomannan substrate, guar gum. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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