Protective effect of Ziziphora clinopodioides flavonoids against H2O2-induced oxidative stress in HUVEC cells
| Autor: | Yanming Wang, Yuche Wu, Xin-Hua Nabi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Programmed cell death Apoptosis RM1-950 medicine.disease_cause Superoxide dismutase 03 medical and health sciences 0302 clinical medicine HUVEC cells medicine MTT assay Viability assay Protein kinase B Pharmacology chemistry.chemical_classification biology Glutathione peroxidase General Medicine Hydrogen peroxide Molecular biology 030104 developmental biology chemistry Oxidative stress 030220 oncology & carcinogenesis biology.protein Therapeutics. Pharmacology |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 117, Iss, Pp-(2019) |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2019.109156 |
| Popis: | The present study was designed to study the protective effect of Ziziphora clinopodioides flavonoids (ZCF) against H2O2-induced oxidative stress in HUVEC cells. MTT assay was carried out to determine the cell viability of HUVEC cells following pretreatment with ZCF. Fluorescent microscopy measurements were performed to evaluate apoptosis of HUVEC cells. Furthermore, the effects of ZCF on the activities of antioxidants superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), malondialdehyde production (MDA) and lactic dehydrogenase (LDH) levels were analyzed. Apoptosis was observed by Hoechst33258 staining and AO staining. Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of B-cell lymphoma/leukemia-2 (Bcl-2), Bcl-2-associated X protein (Bax) and aspartate proteolytic enzyme-3 (Caspase-3) mRNA. The expression of vascular endothelial growth factor receptor 2 (VEGFR2), protein kinase B (Akt), phosphorylated protein kinase B (p-Akt), Bax, Bcl-2 and Caspase-3 were detected by western blot. ZCF attenuated H2O2-induced cell death, as determined by the MTT assay. ZCF decreased malondialdehyde and lactic dehydrogenase levels, increased superoxide dismutase, glutathione peroxidase, catalase activities and inhibited apoptosis. Moreover, pretreatment with ZCF decreased the expression of Bax and Caspase-3 at mRNA level, increased the expression of Bcl-2 mRNA level, decreased the levels of VEGFR2, Bax and Caspase-3 protein, and increased the level of p-Akt / Akt and Bcl-2 protein in HUVEC cells. These results suggested that ZCF protected against H2O2-induced injury in HUVEC cells. The mechanism for this effect is related to the enhancement of antioxidant capacity, suppression of angiogenesis and apoptosis. |
| Databáze: | OpenAIRE |
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