Infrared Spectroscopic Characterization of the Interaction of Cationic Lipids with Plasmid DNA
| Autor: | C. Russell Middaugh, Gary S. Koe, Janet G. Smith, Christopher M. Wiethoff, Thomas J. Anchordoquy, Sirirat Choosakoonkriang |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Glycerophospholipids
Vibration Biochemistry Nucleobase chemistry.chemical_compound Bromide Spectroscopy Fourier Transform Infrared Zeta potential Organic chemistry Colloids Fourier transform infrared spectroscopy Lipid bilayer Molecular Biology Chemistry Phosphatidylethanolamines Cationic polymerization DNA Cell Biology Lipids Cholesterol Membrane Biophysics Nucleic Acid Conformation lipids (amino acids peptides and proteins) Plasmids |
| Zdroj: | Journal of Biological Chemistry. 276:8037-8043 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m010592200 |
| Popis: | Fourier transform infrared spectroscopy was used to characterize the interaction of the cationic lipids 1,2-dioleoyl-3-trimethylammonium-propane and dioctadecyldimethylammonium bromide with plasmid DNA. The effect of incorporating the neutral colipids cholesterol and dioleoylphosphatidylethanolamine on this interaction was also examined. Additionally, dynamic and phase analysis light scattering were used to monitor the size and zeta potential of the resulting complexes under conditions similar to the Fourier transform infrared measurements. Results suggest that upon interaction of cationic lipids with DNA, the DNA remains in the B form. Distinct changes in the frequency of several infrared bands arising from the DNA bases, however, suggest perturbation of their hydration upon interaction with cationic lipids. A direct interaction of the lipid ammonium headgroup with and dehydration of the DNA phosphate is observed when DNA is complexed with these lipids. Changes in the apolar regions of the lipid bilayer are minimal, whereas the interfacial regions of the membrane show changes in hydration or molecular packing. Incorporation of helper lipids into the cationic membranes results in increased conformational disorder of the apolar region and further dehydration of the interfacial region. Changes in the hydration of the DNA bases were also observed as the molar ratio of helper lipid in the membranes was increased. |
| Databáze: | OpenAIRE |
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