Resveratrol-mediated SIRT1 activation attenuates ovalbumin-induced allergic rhinitis in mice
| Autor: | Ruipei Wang, Jian Li, Yingying Luo, Bin Wang, Yajie Bian, Qin Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
biology business.industry Immunology Mucous membrane of nose Inflammation respiratory system Resveratrol Immunoglobulin E Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound Ovalbumin 030104 developmental biology 0302 clinical medicine chemistry biology.protein TLR4 Medicine Nasal Lavage Fluid medicine.symptom business Molecular Biology 030215 immunology |
| Zdroj: | Molecular immunology. 122 |
| ISSN: | 1872-9142 |
| Popis: | Resveratrol (RSV) is one kind of polyphenol that possesses anti-inflammatory effect. The effect of RSV in allergic rhinitis (AR) remains unclear. In this study, we investigated the protective effect of RSV against AR and explored the related signaling pathways. AR mouse model was successfully established by intraperitoneal injection with Ovalbumin (OVA). RSV administration was performed in a diet with different doses (5, 30, 50 mg/kg). Allergic symptoms were indicated by sneezing and nasal rubbing. Cytokines in serum and nasal lavage fluid (NLF) were determined by ELISA. mRNA and protein levels were detected by RT-PCR and Western blot. Our results show that RSV treatment attenuated sneezing and nasal rubbing in AR mice. It decreased histamine release, OVA-specific IgE, IgG1, IL-4 and LTC4, inflammatory cell numbers (leucocytes, eosinophils, lymphocytes, and neutrophils), and inflammatory cytokines secretion (TNF-α, IL-6, IL-10, IL-5, IL-13, and IL-17). Mechanically, RSV treatment suppressed HMGB1 and TLR4 expression and promoted SIRT1 expression in the nasal mucosa. In conclusion, RSV is able to attenuate OVA-induced AR in mice. |
| Databáze: | OpenAIRE |
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