Identification of a novel nidovirus as a potential cause of large scale mortalities in the endangered Bellinger River snapping turtle (Myuchelys georgesi)
| Autor: | Xingnian Gu, Sarah Gestier, AJ Read, Jing Zhang, Melinda J Frost, Cheryl Jenkins, Mukesh Srivastava, Peter D. Kirkland, Karrie Rose, Deborah S. Finlaison, Kate Parrish, Jane Hall |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Range (biology) Endangered species lcsh:Medicine Nidovirales Negative Staining law.invention 0403 veterinary science Database and Informatics Methods law Medicine and Health Sciences Turtle (robot) Pythons lcsh:Science Phylogeny Staining education.field_of_study Multidisciplinary Myuchelys georgesi Eukaryota Cell Staining Lizards Snakes 04 agricultural and veterinary sciences Squamates Turtles Real-time polymerase chain reaction Vertebrates RNA Viral Biological Cultures Anatomy Sequence Analysis Research Article Bioinformatics 040301 veterinary sciences Population Zoology Biology Research and Analysis Methods Virus 03 medical and health sciences Rivers Animals education Endangered Species lcsh:R Australia Organisms Biology and Life Sciences Reptiles Outbreak Kidneys Renal System Cell Cultures biology.organism_classification 030104 developmental biology Specimen Preparation and Treatment Testudines Amniotes lcsh:Q Sequence Alignment |
| Zdroj: | PLoS ONE, Vol 13, Iss 10, p e0205209 (2018) PLOS ONE PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | In mid-February 2015, a large number of deaths were observed in the sole extant population of an endangered species of freshwater snapping turtle, Myuchelys georgesi, in a coastal river in New South Wales, Australia. Mortalities continued for approximately 7 weeks and affected mostly adult animals. More than 400 dead or dying animals were observed and population surveys conducted after the outbreak had ceased indicated that only a very small proportion of the population had survived, severely threatening the viability of the wild population. At necropsy, animals were in poor body condition, had bilateral swollen eyelids and some animals had tan foci on the skin of the ventral thighs. Histological examination revealed peri-orbital, splenic and nephric inflammation and necrosis. A virus was isolated in cell culture from a range of tissues. Nucleic acid sequencing of the virus isolate has identified the entire genome and indicates that this is a novel nidovirus that has a low level of nucleotide similarity to recognised nidoviruses. Its closest relatives are nidoviruses that have recently been described in pythons and lizards, usually in association with respiratory disease. In contrast, in the affected turtles, the most significant pathological changes were in the kidneys. Real time PCR assays developed to detect this virus demonstrated very high virus loads in affected tissues. In situ hybridisation studies confirmed the presence of viral nucleic acid in tissues in association with pathological changes. Collectively these data suggest that this virus is the likely cause of the mortalities that now threaten the survival of this species. Bellinger River Virus is the name proposed for this new virus. |
| Databáze: | OpenAIRE |
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