Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Actinobacillus pleuropneumoniae based the dsbE-like gene
| Autor: | Haitao Li, Yin Wang, Hongwei Ji, Wanzhu Guo, Zhiwen Xu, Zhicai Zuo, Hui Zhang, Ling Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Bordetella bronchiseptica
lcsh:Veterinary medicine General Veterinary biology animal diseases Actinobacillus pleuropneumoniae Loop-mediated isothermal amplification Streptococcus suis respiratory system biology.organism_classification Porcine reproductive and respiratory syndrome virus bacterial infections and mycoses sensitivity Virology Microbiology respiratory tract diseases Salmonella enterica Haemophilus lcsh:SF600-1100 loop-mediated isothermal amplification (LAMP) Pasteurella multocida |
| Zdroj: | Pesquisa Veterinária Brasileira, Vol 32, Iss 8, Pp 757-760 (2012) Pesquisa Veterinária Brasileira v.32 n.8 2012 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Volume: 32, Issue: 8, Pages: 757-760, Published: AUG 2012 |
| ISSN: | 1678-5150 |
| Popis: | This paper reports on the development and validation of a loop-mediated isothermal amplification assay (LAMP) for the rapid and specific detection of Actinobacillus pleuropneumoniae (A. pleuropneumoniae). A set of six primers were designed derived from the dsbE-like gene of A.pleuropneumoniae and validate the assay using 9 A. pleuropneumoniae reference/field strains, 132 clinical isolates and 9 other pathogens. The results indicated that positive reactions were confirmed for all A. pleuropneumoniae strains and specimens by LAMP at 63ºC for 60 min and no cross-reactivity were observed from other non-A.pleuropneumoniae including Haemophilus parasuis, Escherichia coli, Pasteurella multocida, Bordetella bronchiseptica, Streptococcus suis, Salmonella enterica, Staphylococcus, porcine reproductive and respiratory syndrome virus (PRRSV), and Pseudorabies virus. The detection limit of the conventional PCR was 10² CFU per PCR test tube, while that of the LAMP was 5 copies per tube. Therefore, the sensitivity of LAMP was higher than that of PCR. Moreover, the LAMP assay provided a rapid yet simple test of A. pleuropneumoniae suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction. |
| Databáze: | OpenAIRE |
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