Analysis of xysA, a gene from Streptomyces halstedii JM8 that encodes a 45-kilodalton modular Xylanase, Xys1
| Autor: | Pablo Sánchez, Ramón I. Santamaría, M Raida, Juan J. Calvete, Alberto Ruiz-Arribas, José M. Fernández-Abalos |
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Transcription
Genetic Blotting Western Molecular Sequence Data Gene Expression Sequence alignment Biology Applied Microbiology and Biotechnology Streptomyces Bacterial Proteins Gene expression Amino Acid Sequence Cellulose Peptide sequence chemistry.chemical_classification Recombination Genetic Ecology Sequence Homology Amino Acid Streptomycetaceae Nucleic acid sequence Chromosome Mapping Sequence Analysis DNA biology.organism_classification Amino acid Culture Media Xylan Endo-1 3-beta-Xylosidase Xylosidases Biochemistry chemistry Xylanase Xylans Sequence Alignment Food Science Biotechnology Research Article Plasmids |
| Zdroj: | Scopus-Elsevier ResearcherID CIÊNCIAVITAE Europe PubMed Central |
| Popis: | The gene xysA from Streptomyces halstedii JM8 encodes a protein of 461 amino acids (Xys1) which is secreted into the culture supernatant as a protein of 45 kDa (Xys1L). Later, this form is proteolytically processed after residue D-362 to produce the protein Xys1S, which conserves the same xylanolytic activity. The cleavage removes a domain of 99 amino acids that shows similarity to bacterial cellulose binding domains and that allows the protein Xys1L to bind to crystalline cellulose (Avicel). Expression of this monocistronic gene is affected by the carbon source present in the culture medium, xylan being the best inducer. By using an anti-Xys1L serum, we have been able to detect xylanases similar in size to Xys1L and Xys1S in most of the different Streptomyces species analyzed, suggesting the ubiquity of these types of xylanases and their processing mechanism. |
| Databáze: | OpenAIRE |
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