Analysis of the protease sequences of HIV-1 infected individuals after Indinavir monotherapy
| Autor: | Amilcar Tanuri, Ana Paula de C Guimarães, Carlos Fernando Barreto de Oliveira, Ricardo Sobhie Diaz, Luciana Jesus Costa, Dercy Jose de Sa-Filho, Conceicao A. Accetturi |
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| Rok vydání: | 2003 |
| Předmět: |
Nonsynonymous substitution
Genotype medicine.medical_treatment HIV Infections Indinavir Drug resistance Biology HIV Protease Polymorphism (computer science) Virology Genetic variation medicine Humans Phylogeny chemistry.chemical_classification Protease Sequence Homology Amino Acid Genetic Variation HIV Protease Inhibitors Infectious Diseases Enzyme chemistry DNA Viral HIV-1 Viral load medicine.drug |
| Zdroj: | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 28(2) |
| ISSN: | 1386-6532 |
| Popis: | Background: Protease inhibitors (PI) are an important HIV-1 treatment tool. The HIV-1 genetic diversity as a result of antiretroviral exposure is a potential barrier to successful antiretroviral therapy. Objectives: To describe the impact of the selective pressure of the PI Indinavir in the protease region of the pol gene of HIV-1. Study design: We have examined the extent of protease sequence heterogeneity in previously antiretroviral drug naive HIV-1 infected individuals receiving Indinavir as monotherapy for at least 48 weeks. Results: Analysis based on the consensus of this group of sequences showed regions with higher and lower polymorphism. The degree of genetic variation was greater in regions less critical for the structure and function of the enzyme. To investigate the selective pressure imposed by drug therapy, we have analyzed the rate of synonymous (ds) and nonsynonymous (dn) substitutions. The three critical regions for enzyme activity showed ds/dn ratio >1. whereas other regions had ds/dn ratio |
| Databáze: | OpenAIRE |
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