Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in
| Autor: | Xi Liu, Suning Liu, Subba Reddy Palli, Yue Gao, Weiting Chen, Kang Li, Sheng Li, Wei Ge, Qili Feng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
media_common.quotation_subject medicine.medical_treatment 20-Hydroxyecdysone Kruppel-Like Transcription Factors Biology 03 medical and health sciences chemistry.chemical_compound Corpora Allata medicine Animals Drosophila Proteins hormone biosynthesis Metamorphosis media_common Multidisciplinary antagonistic action juvenile hormone fungi Metamorphosis Biological Gene Expression Regulation Developmental Biological Sciences Prothoracic gland Cell biology Juvenile Hormones Steroid hormone 030104 developmental biology Drosophila melanogaster Ecdysterone chemistry Juvenile hormone ring gland Corpus allatum 20-hydroxyecdysone Ecdysone Hormone Signal Transduction Developmental Biology |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America |
| ISSN: | 1091-6490 |
| Popis: | Significance In vertebrates, steroid hormones regulate developmental transition from juveniles to adults. Insect steroid hormone, 20-hydroxyecdysone (20E), coordinates with juvenile hormone (JH) to regulate metamorphosis; however, the precise cross-talk mechanism is not well understood. Here, we report that JH and 20E antagonize each other’s biosynthesis in a major endocrine organ of Drosophila larvae: JH suppresses ecdysone biosynthesis and inhibits metamorphosis, whereas 20E suppresses JH biosynthesis and promotes metamorphosis. These data answer a long-standing question on how the mutual antagonism between the two major insect hormones regulates metamorphosis and may help to understand the hormonal regulation of developmental transition in mammals. In both vertebrates and insects, developmental transition from the juvenile stage to adulthood is regulated by steroid hormones. In insects, the steroid hormone, 20-hydroxyecdysone (20E), elicits metamorphosis, thus promoting this transition, while the sesquiterpenoid juvenile hormone (JH) antagonizes 20E signaling to prevent precocious metamorphosis during the larval stages. However, not much is known about the mechanisms involved in cross-talk between these two hormones. In this study, we discovered that in the ring gland (RG) of Drosophila larvae, JH and 20E control each other’s biosynthesis. JH induces expression of a Krüppel-like transcription factor gene Kr-h1 in the prothoracic gland (PG), a portion of the RG that produces the 20E precursor ecdysone. By reducing both steroidogenesis autoregulation and PG size, high levels of Kr-h1 in the PG inhibit ecdysteriod biosynthesis, thus maintaining juvenile status. JH biosynthesis is prevented by 20E in the corpus allatum, the other portion of the RG that produces JH, to ensure the occurrence of metamorphosis. Hence, antagonistic actions of JH and 20E within the RG determine developmental transitions in Drosophila. Our study proposes a mechanism of cross-talk between the two major hormones in the regulation of insect metamorphosis. |
| Databáze: | OpenAIRE |
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