Light-induced triplet-triplet electron resonance spectroscopy
| Autor: | Alice M. Bowen, Marina Gobbo, Marta De Zotti, Arnau Bertran, Marilena Di Valentin, Christiane R. Timmel, Kevin B. Henbest |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Letter
Materials science PULSE EPR DEER 02 engineering and technology 010402 general chemistry NUCLEAR DOUBLE-RESONANCE CHLOROPHYLL A-PROTEIN DISTANCE MEASUREMENTS ORIENTATION SELECTION PULSE EPR STATE DEER PORPHYRIN BIOMACROMOLECULES MICROSCOPY 01 natural sciences NUCLEAR DOUBLE-RESONANCE BIOMACROMOLECULES law.invention Paramagnetism law Moiety General Materials Science Physical and Theoretical Chemistry Spectroscopy Electron paramagnetic resonance ORIENTATION SELECTION DISTANCE MEASUREMENTS MICROSCOPY 021001 nanoscience & nanotechnology STATE 0104 chemical sciences Dipole Förster resonance energy transfer Chemical physics PORPHYRIN CHLOROPHYLL A-PROTEIN Density functional theory 0210 nano-technology Macromolecule |
| Zdroj: | The Journal of Physical Chemistry Letters |
| Popis: | We present a new technique, light-induced triplet-triplet electron resonance spectroscopy (LITTER), which measures the dipolar interaction between two photoexcited triplet states, enabling both the distance and angular distributions between the two triplet moieties to be determined on a nanometer scale. This is demonstrated for a model bis-porphyrin peptide that renders dipolar traces with strong orientation selection effects. Using simulations and density functional theory calculations, we extract distance distributions and relative orientations of the porphyrin moieties, allowing the dominant conformation of the peptide in a frozen solution to be identified. LITTER removes the requirement of current light-induced electron spin resonance pulse dipolar spectroscopy techniques to have a permanent paramagnetic moiety, becoming more suitable for in-cell applications and facilitating access to distance determination in unmodified macromolecular systems containing photoexcitable moieties. LITTER also has the potential to enable direct comparison with Förster resonance energy transfer and combination with microscopy inside cells. |
| Databáze: | OpenAIRE |
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