Identification of Paracrine Neuroprotective Candidate Proteins by a Functional Assay-driven Proteomics Approach
| Autor: | Stephanie Schoeffmann, Karsten Boldt, Stefanie M. Hauck, Per Ekström, Christian Johannes Gloeckner, Margaret E. Harley, Marius Ueffing |
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| Rok vydání: | 2008 |
| Předmět: |
Proteomics
IGFBP7 Cell Survival Swine medicine.medical_treatment Nerve Tissue Proteins Biology Cell Fractionation Eye Biochemistry Analytical Chemistry Paracrine signalling Neurotrophic factors Paracrine Communication medicine Animals Humans Photoreceptor Cells Molecular Biology Anion Exchange Resins Cells Cultured Growth factor Biological activity Molecular biology Cell biology CTGF Neuroprotective Agents Culture Media Conditioned biology.protein Feasibility Studies Neuroglia Algorithms Neurotrophin |
| Zdroj: | Mol. Cell. Proteomics 7, 1349-1361 (2008) |
| ISSN: | 1535-9476 |
| DOI: | 10.1074/mcp.m700456-mcp200 |
| Popis: | Glial cells support neuronal survival and function by secreting neurotrophic cytokines. Retinal Mueller glial cells (RMGs) support retinal neurons, especially photoreceptors. These highly light-sensitive sensory neurons receive vision, and their death results in blinding diseases. It has been proposed that RMGs release factors that support photoreceptor survival, but the nature of these factors remains to be elucidated. To discover such neurotrophic factors, we developed an integrated work flow toward systematic identification of neuroprotective proteins, which are, like most cytokines, expressed only in minute amounts. This strategy can be generally applied to identify secreted bioactive molecules from any body fluid once a recipient cell for this activity is known. Toward this goal we first isolated conditioned medium (CM) from primary porcine RMGs cultured in vitro and tested for survival-promoting activity using primary photoreceptors. We then developed a large scale, microplate-based cellular high content assay that allows rapid assessment of primary photoreceptor survival concomitant with biological activity in vitro. The enrichment strategy of bioactive proteins toward their identification consists of several fractionation steps combined with tests for biological function. Here we combined 1) size fractionation, 2) ion exchange chromatography, 3) reverse phase liquid chromatography, and 4) mass spectrometry (Q-TOF MS/MS or MALDI MS/MS) for protein identification. As a result of this integrated work flow, the insulin-like growth factor-binding proteins IGFBP5 and IGFBP7 and connective tissue growth factor (CTGF) were identified as likely candidates. Cloning and stable expression of these three candidate factors in HEK293 cells produced conditioned medium enriched for either one of the factors. IGFBP5 and CTGF, but not IGFBP7, significantly increased photoreceptor survival when secreted from HEK293 cells and when added to the original RMG-CM. This indicates that the survival-promoting activity in RMG-CM is multifactorial with IGFBP5 and CTGF as an integral part of this activity. |
| Databáze: | OpenAIRE |
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