Expression profile and estrogenic regulation of Amh during gonadal sex differentiation in northern snakehead (Channa argus)
| Autor: | Mi Ou, Haiyang Liu, Qing Luo, Kunci Chen, Lanlan Zhang, Yanduo Wu, Jian Zhao, Dandan Gao |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Anti-Mullerian Hormone Male endocrine system Channa argus Sex Differentiation medicine.drug_class Ethinyl Estradiol 01 natural sciences Biochemistry Snakehead Andrology 03 medical and health sciences Exon Testis Genetics medicine Animals Molecular Biology Sexual differentiation biology Ovary Fishes Gene Expression Regulation Developmental biology.organism_classification Sexual dimorphism 030104 developmental biology Estrogen Female Development of the gonads 010606 plant biology & botany Hormone |
| Zdroj: | Genesgenomics. 42(7) |
| ISSN: | 2092-9293 |
| Popis: | Anti-Mullerian hormone (Amh) plays a critical role in both early sex determination and later gonad development in vertebrate species. However, it remains unknown in northern snakehead (Channa argus), which is economically important freshwater fish with sexual dimorphism. This study aimed to identify the expression profiles and estrogenic regulation of CaAmh during gonadal sex differentiation in C. argus. The cDNA and genomic DNA sequences of CaAmh were identified by PCR and RACE techniques. The expression patterns of CaAmh were detected by qRT-PCR during the gonadal sex differentiation and after 17α-ethinyloestradiol (EE2) treatments. CaAmh is composed of seven exons and six introns, and its full-length cDNA is 2413 bp in length, with 1635 bp open reading frame (ORF) that encodes a 544 amino acid protein. Tissues expression patterns revealed that CaAmh display the highest expression in testis of XY males (40.36 folds, p |
| Databáze: | OpenAIRE |
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